| Huang GC |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=1.639 ------>paper_class3=2 ------>paper_class2=1 ------>vol=71 ------>confirm_bywho=kyhsu ------>insert_bywho=crystal ------>Jurnal_Rank=26.8 ------>authors4_c=None ------>comm_author=1 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=224 ------>medlineContent= ------>unit=G0100 ------>insert_date=20041113 ------>iam=4 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Planta Medica. ------>paper_name=Antitumor Effects of Zerumbone from Zingiber zerumbet in P-388D1 Cells in Vitro and in Vivo ------>confirm_date=20051220 ------>tch_id=088003 ------>pmid=15770541 ------>page1=219 ------>fullAbstract=The fresh rhizome of Zingiber zerumbet (L.) Roscoe ex Smith (Zingiberaceae) is widely used as a folk medicine in Taiwan. In this study, the fresh rhizome was extracted with 95 % EtOH and partitioned with diethyl ether. The antitumor effects of the diethyl ether extract were measured in cultured P-388D (1) cells and in an animal model of P-388D (1)-bearing CDF (1) mice. The results indicated that the extract could induce DNA fragmentation in P-388D (1) cells in vitro, and significantly prolong the life of P-388D (1)-bearing CDF (1) mice (ILS% = 127.8) at a dosage of 5 mg/kg body weight. After column chromatography combined with an MTT cytotoxicity bioassay, zerumbone, a cyclic sesquiterpene was isolated from the diethyl ether extract. Zerumbone inhibited the growth of P-388D (1) cells, induced DNA fragmentation in culture, and significantly prolonged the life of P-388D (1)-bearing CDF (1) mice (ILS% = 120.5) at a dosage of 2 mg/kg. Furthermore, zerumbone inhibited the growth of a human leukemia cell line, HL-60 cells, in a time- and concentration-dependent manner, with IC (50) values of 22.29, 9.12, and 2.27 microg/mL for 6, 12, and 18 h, respectively. The cell cycle of HL-60 cells was observed after treatment with zerumbone, which induced G (2)/M cell cycle arrest in HL-60 cells in a time- and concentration-dependent manner, and decreased the cyclin B1/cdk 1 protein level. These results suggest that zerumbone is an active principal of Z. zerumbet and is potentially a lead compound for the development of anticancer drugs. ------>tmu_sno=None ------>sno=10335 ------>authors2=Chein TY ------>authors3=Chen LG ------>authors4=Wang CC ------>authors5= ------>authors6= ------>authors6_c=None ------>authors=Huang GC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Antitumor effects of zerumbone from Zingiber zerumbet in P-388D1 cells in vitro and in vivo. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=None |