| Chen TH |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=1042 ------>confirm_bywho=shiemin ------>insert_bywho=tsohsiao ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=93 ------>medlineContent= ------>unit=E0100 ------>insert_date=20050731 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=None ------>authors_c=None ------>score=444 ------>journal_name=Ann N Y Acad Sci. ------>paper_name=Angiotensin II Stimulates Hypoxia-Inducible Factor 1{alpha} Accumulation in Glomerular Mesangial Cells. ------>confirm_date=20050817 ------>tch_id=090107 ------>pmid=15965074 ------>page1=286 ------>fullAbstract=Hypoxia increases hypoxia-inducible factor 1alpha (HIF-1alpha) protein levels by inhibiting ubiquitination and degradation of HIF-1alpha, which regulates the transcription of many genes. Recent studies have revealed that many ligands can stimulate HIF-1alpha accumulation under nonhypoxic conditions. In this study, we show that angiotensin II (Ang II) increased HIF-1alpha protein levels in a time- and dose-dependent manner under normoxic conditions. Treatment of mesangial cells with Ang II (100 nM) increased production of reactive oxygen species (ROS). Ang II (100 nM) increased the phosphorylation of PDK-1 and Akt/PKB in glomerular mesangial cells. Ang II-stimulated HIF-1alpha accumulation was blocked by the phosphatidylinositol 3-kinase (PI-3K) inhibitors, Ly 294001, and wortmannin, suggesting that PI-3K was involved. Because increased ROS generation by Ang II may activate the PI-3K-PKB/Akt signaling pathway, these results suggest that Ang II may stimulate a ROS-dependent activation of the PI-3K-PKB/Akt pathway, which leads to HIF-1alpha accumulation. ------>tmu_sno=None ------>sno=11625 ------>authors2=Wang JF ------>authors3=Chan P ------>authors4=Lee HM ------>authors5= ------>authors6= ------>authors6_c=None ------>authors=Chen TH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Angiotensin II stimulates hypoxia-inducible factor 1alpha accumulation in glomerular mesangial cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=None |