| Fu SL |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=2.904 ------>paper_class3=2 ------>paper_class2=1 ------>vol=339 ------>confirm_bywho=hsu0320 ------>insert_bywho=wchou ------>Jurnal_Rank=35.9 ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=144 ------>medlineContent= ------>unit=G0300 ------>insert_date=20051130 ------>iam=4 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Biochem. Biophys. Res. Communi. ------>paper_name=dioscorin isolated from Dioscorea alata activates TLR4-signaling pathways and induces cytokine expression in macrophages ------>confirm_date=20061229 ------>tch_id=089063 ------>pmid=16297883 ------>page1=137 ------>fullAbstract=The Toll-like receptor 4 (TLR4)-signaling pathway is crucial for activating both innate and adaptive immunity. TLR4 is a promising molecular target for immune-modulating drugs, and TLR4 agonists are of therapeutic potential for treating immune diseases and cancers. Several medicinal herb-derived components have recently been reported to act via TLR4-dependent pathways, suggesting that medicinal plants are potential resources for identifying TLR4 activators. We have applied a screening procedure to systematically identify herbal constituents that activate TLR4. To exclude possible LPS contamination in these plant-derived components, a LPS inhibitor, polymyxin B, was added during screening. One of the plant components we identified from the screening was dioscorin, the glycoprotein isolated from Dioscorea alata. It induced TLR4-downstream cytokine expression in bone marrow cells isolated from TLR4-functional C3H/HeN mice but not from TLR4-defective C3H/HeJ mice. Dioscorin also stimulated multiple signaling molecules (NF-kappaB, ERK, JNK, and p38) and induced the expression of cytokines (TNF-alpha, IL-1beta, and IL-6) in murine RAW 264.7 macrophages. Furthermore, the ERK, p38, JNK, and NF-kappaB-mediated pathways are all involved in dioscorin-mediated TNF-alpha production. In summary, our results demonstrate that dioscorin is a novel TLR4 activator and induces macrophage activation via typical TLR4-signaling pathways. ------>tmu_sno=None ------>sno=11913 ------>authors2=Hsu YH ------>authors3=Lee PY ------>authors4=Hou WC ------>authors5=Hung LC ------>authors6=Lin CH, Chen CM, Huang YJ ------>authors6_c=None ------>authors=Fu SL ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Dioscorin isolated from Dioscorea alata activates TLR4-signaling pathways and induces cytokine expression in macrophages. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=None |