| Chen CH |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=2.432 ------>paper_class3=2 ------>paper_class2=1 ------>vol=4 ------>confirm_bywho=ncchang ------>insert_bywho=tsohsiao ------>Jurnal_Rank=32.1 ------>authors4_c=None ------>comm_author=1 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=15 ------>medlineContent= ------>unit=E0109 ------>insert_date=20060125 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Eur J Pharmacol ------>paper_name=The protective effect of prostacyclin on adriamycin-induced apoptosis in rat renal tubular cells ------>confirm_date=20060125 ------>tch_id=090107 ------>pmid=16343480 ------>page1=8 ------>fullAbstract=Adriamycin-induced nephrosis in rats is a commonly used experimental model for pharmacological studies of human chronic renal diseases. Adriamycin-induced apoptosis of renal tubular cells has been reported in adriamycin-treated rats. In addition, prostacyclin (PGI(2)) is known to have various protective effects on many kinds of cells. To investigate the protective effect of PGI(2) on cells undergoing adriamycin-induced apoptosis, this study selectively augmented PGI(2) production via adenovirus-mediated transfer of genes for cyclooxygenase-1 (COX-1) and prostacyclin synthase (PGIS) (two key enzymes of PGI(2) synthesis) to renal tubular cells. This PGI(2) overexpression protected rat renal tubular cells from adriamycin-induced apoptosis. Ad-COX-1/PGIS transfection was found to reduce the adriamycin-stimulated activities of caspase-3 and caspase-9, inhibit adriamycin-induced release of cytochrome c, elevate the expression of Bcl-x(L), and suppress the activation and translocation of nuclear factor-kappaB (NF-kappaB) in adriamycin-treated renal tubular cells. Our results reveal that selective augmentation of PGI(2) production can protect rat renal tubular cells from adriamycin-induced apoptosis via the NF-kappaB signaling pathway. This implies the therapeutic potential of combined COX-1 and PGIS gene transfer in gene therapy for chronic renal diseases. ------>tmu_sno=None ------>sno=12448 ------>authors2=Lin H ------>authors3=Hsu YH ------>authors4=Sue YM ------>authors5=Cheng TH ------>authors6=Chan P, Chen TH ------>authors6_c=None ------>authors=Chen CH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=The protective effect of prostacyclin on adriamycin-induced apoptosis in rat renal tubular cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=529 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=None |