| Wei HJ |
------>authors3_c=None ------>paper_class1=2 ------>Impact_Factor=None ------>paper_class3=0 ------>paper_class2=0 ------>vol= ------>confirm_bywho=tzengcr ------>insert_bywho=tzengcr ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=5 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0111 ------>insert_date=20060221 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c=None ------>score=-86 ------>journal_name=Presented at 1998 Annual Meeting of the Chinese Fertility Society. ------>paper_name=Genetic diagnosis in male infertility. ------>confirm_date=20060228 ------>tch_id=079009 ------>pmid=19697700 ------>page1= ------>fullAbstract=Immunocontraceptive technology is beginning to attract more attention for the control of wild mouse or other pest animals. Oral vaccination of animals and man, to provide effective mucosal and/or systemic immunity, is a good delivery system, but it remains poor bioavailablity, one of the most promising strategies to improve the oral delivery relies on polymeric nanoparticles carriers to encapsulate vaccine. Nanoemulsion was used as the carriers of oral immunization of Lugurus Lugurus zona pellucida 3, the immune response and antifertility potential of this vaccine is valuated. Nanoemulsion-encapsulated plasmid pcDNA3.0-Aat-COMP-lzp3-C3d3 (pACLC DNA vaccine) was prepared and evaluated the encapsulation efficiency by anion exchange chromatography. The mice were orally delivered with nanoemulsion-encapsulated plasmid, to detect specific IgG in serum and IgA in vaginal fluid by indirect ELISA, and to perform fertility experiment, to do histological analysis of immunized mice ovary. The results showed that the specific IgG and IgA antibody is elicited, the fertility rate is deceased at a certain extent. In conclusion, it is feasible that nanoemulsion is used as the carriers of oral immunization. ------>tmu_sno=None ------>sno=13054 ------>authors2=Chang JG ------>authors3=Chiang HS ------>authors4=Lin WM ------>authors5=Sung CY ------>authors6=Tzeng CR ------>authors6_c=None ------>authors=Wei HJ ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=1 ------>updateTitle=[Oral immunization of Lugurus lugurus with the plasmid nanoemulsion of zona pellucida 3 to enhance infertility effects] ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=1998 ------>submit_flag=None ------>publish_month=9 |