Chang CC |
------>authors3_c=??? ------>paper_class1=2 ------>Impact_Factor=None ------>paper_class3=0 ------>paper_class2=0 ------>vol= ------>confirm_bywho=cmshih ------>insert_bywho=rita1204 ------>Jurnal_Rank=None ------>authors4_c=??? ------>comm_author=1 ------>patent_EDate=None ------>authors5_c=??? ------>publish_day=19 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0103 ------>insert_date=20060330 ------>iam=5 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c=??? ------>score=71 ------>journal_name=The 21th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan (O18).():-. ------>paper_name=Mouse germ line stem cells ------>confirm_date=20060331 ------>tch_id=090143 ------>pmid=19906868 ------>page1= ------>fullAbstract=The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation. To explore the function of Nanog in germ cells, Nanog RNA was conditionally knocked down in vivo by shRNA. Nanog shRNA transgenic (NRi-Tg) mice were generated through the formation of germline chimeras with NRi-Tg embryonic stem cells. In E12.5 Cre-induced ER-Cre/NRi-Tg and TNAP-Cre/NRi-Tg double-transgenic embryos, the number of alkaline phosphatase-positive and SSEA1-positive PGCs decreased significantly. In the E9.5 and E10.5 migrating Nanog-knockdown PGCs, TUNEL-positive apoptotic cell death became prominent in vivo and in vitro, despite Oct4 expression. Single-cell microarray analysis of E10.5 Nanog-knockdown PGCs revealed significant up- and downregulation of a substantial number of genes, including Tial1, Id1 and Suz12. These data suggest that Nanog plays a key role in the proliferation and survival of migrating PGCs as a safeguard of the PGC-specific molecular network. ------>tmu_sno=None ------>sno=13224 ------>authors2=Wu TJ ------>authors3=Chou ML ------>authors4=Hsu JA ------>authors5=Huang YH ------>authors6= ------>authors6_c= ------>authors=Chang CC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=1 ------>updateTitle=Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=3 |