Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Chang CC
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------>insert_date=20060330
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------>journal_name=The 21th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan (O18).():-.
------>paper_name=Mouse germ line stem cells
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------>fullAbstract=The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation. To explore the function of Nanog in germ cells, Nanog RNA was conditionally knocked down in vivo by shRNA. Nanog shRNA transgenic (NRi-Tg) mice were generated through the formation of germline chimeras with NRi-Tg embryonic stem cells. In E12.5 Cre-induced ER-Cre/NRi-Tg and TNAP-Cre/NRi-Tg double-transgenic embryos, the number of alkaline phosphatase-positive and SSEA1-positive PGCs decreased significantly. In the E9.5 and E10.5 migrating Nanog-knockdown PGCs, TUNEL-positive apoptotic cell death became prominent in vivo and in vitro, despite Oct4 expression. Single-cell microarray analysis of E10.5 Nanog-knockdown PGCs revealed significant up- and downregulation of a substantial number of genes, including Tial1, Id1 and Suz12. These data suggest that Nanog plays a key role in the proliferation and survival of migrating PGCs as a safeguard of the PGC-specific molecular network.
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------>authors2=Wu TJ
------>authors3=Chou ML
------>authors4=Hsu JA
------>authors5=Huang YH
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------>authors=Chang CC
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------>updateTitle=Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells.
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------>publish_year=2006
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------>publish_month=3
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z