| Wu LT |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.361 ------>paper_class3=2 ------>paper_class2=1 ------>vol=11 ------>confirm_bywho=shiemin ------>insert_bywho=yuleon03 ------>Jurnal_Rank=36.6 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=897 ------>medlineContent= ------>unit=E0100 ------>insert_date=20060424 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Clin Microbiol Infect ------>paper_name=Identification of a Novel Cephalosporinase (DHA-3) in Klebsiella pneumoniae Isolated in Taiwan. ------>confirm_date=20070421 ------>tch_id=093040 ------>pmid=16216104 ------>page1=893 ------>fullAbstract=A strain of Klebsiella pneumoniae resistant to cefoxitin and oxyimino-cephalosporins, but susceptible to cefepime, was isolated from an adult patient hospitalised in Taichung, Taiwan. Isoelectric focusing revealed three beta-lactamases with isoelectric points of 5.4, 8.2 and 7.9, respectively. Following PCR with plasmid DNA templates and gene sequencing, these enzymes were shown to correspond to TEM-1, SHV-5 and a novel DHA-1-like enzyme (designated DHA-3). The bla genes for TEM-1 and SHV-5 were transferable, but the bla(DHA-3) gene was non-self-transferable in conjugation experiments. All three bla genes were successfully introduced by electrotransformation into an Escherichia coli recipient (DH5alpha), resulting in a similar resistance profile to that observed in the original donor strain. Other K. pneumoniae strains producing DHA-1-like enzymes have been identified previously in Taiwan, and this report suggests that DHA-type beta-lactamases are continuing to emerge in this country. ------>tmu_sno=None ------>sno=13449 ------>authors2=Hung SW ------>authors3=Chuang YC ------>authors4=Chen HE ------>authors5=Jones RN ------>authors6=Yu WL ------>authors6_c= ------>authors=Wu LT ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Identification of a novel cephalosporinase (DHA-3) in Klebsiella pneumoniae isolated in Taiwan. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=11 |