| Chen SD |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.789 ------>paper_class3=2 ------>paper_class2=1 ------>vol=1042 ------>confirm_bywho=ryyuan ------>insert_bywho=hsuc ------>Jurnal_Rank=15.6 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=364 ------>medlineContent= ------>unit=E0100 ------>insert_date=20060426 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Ann. N. Y. Acad. Sci. ------>paper_name=Pravastatin Attenuates Ceramide-Induced Cytotoxicity in Mouse Cerebral Endothelial Cells with HIF-1 Activation and VEGF Upregulation. ------>confirm_date=20060502 ------>tch_id=091141 ------>pmid=15965081 ------>page1=357 ------>fullAbstract=Ceramide is a pro-apoptotic lipid messenger that induces oxidative stress and may mediate apoptosis in cerebral endothelial cells (CECs) induced by TNF-alpha/cycloheximide, lipopolysaccharide, oxidized LDL, IL-1, and amyloid peptide. Exposure of CECs to C2 ceramide for 12 h caused cell death in a concentration-dependent manner, with a LC50 of 30 microM. Statins are inhibitors of 3-hydroxyl-3-methyl coenzyme A reductase which is the rate-limiting enzyme for cholesterol biosynthesis. Pretreatment with pravastatin at 20 microM for 16 h substantially attenuated ceramide cytotoxicity in mouse CECs. Increases in vascular endothelial growth factor (VEGF) expression were detected within 1-3 h after pravastatin treatment. This pravastatin action was accompanied by the activation of hypoxia-inducible factor-1 (HIF-1), a transcription factor known to activate VEGF expression. These results raise the possibility that pravastatin may protect CECs against ceramide-induced death via the HIF-VEGF cascade. ------>tmu_sno=None ------>sno=13459 ------>authors2=Hu CJ ------>authors3=Yang DI ------>authors4=Nassief A ------>authors5=Chen H ------>authors6=Yin K, Xu J, Hsu CY ------>authors6_c= ------>authors=Chen SD ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Pravastatin attenuates ceramide-induced cytotoxicity in mouse cerebral endothelial cells with HIF-1 activation and VEGF upregulation. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=5 |