| Yang DI |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.789 ------>paper_class3=2 ------>paper_class2=1 ------>vol=1042 ------>confirm_bywho=ryyuan ------>insert_bywho=hsuc ------>Jurnal_Rank=15.6 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=447 ------>medlineContent= ------>unit=E0100 ------>insert_date=20060426 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Ann. N. Y. Acad. Sci. ------>paper_name=Antisense RNA to inducible nitric oxide synthase reduces cytokine-mediated brain endothelial cell death. ------>confirm_date=20060502 ------>tch_id=091141 ------>pmid=15965090 ------>page1=439 ------>fullAbstract=We test whether inhibition of inducible nitric oxide synthase (iNOS) can exert a cytoprotective effect on cerebral endothelial cells upon stimulation by pro-inflammatory cytokines. Mouse brain endothelial cells were stably transfected to express an antisense RNA against iNOS driven by an endothelium-specific von Willebrand factor (vWF) promoter. Upon stimulation with tumor necrosis factor-alpha (TNF-alpha) plus interferon-gamma (IFN-gamma), antisense transfectants showed less iNOS enzymatic activity with less nitric oxide (NO) when compared to the sense control cells. Correspondingly, the antisense cells showed a reduced LDH release and less cytosolic content of oligonucleosomes. These findings establish a cell-specific antisense strategy and confirm the cytotoxic role of iNOS expression in cultured cerebral endothelial cells. ------>tmu_sno=None ------>sno=13460 ------>authors2=Chen S ------>authors3=Ezekiel UR ------>authors4=Xu J ------>authors5=Wu Y ------>authors6=Hsu CY ------>authors6_c= ------>authors=Yang DI ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Antisense RNA to inducible nitric oxide synthase reduces cytokine-mediated brain endothelial cell death. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=5 |