Chen HI |
------>authors3_c=??? ------>paper_class1=1 ------>Impact_Factor=4.182 ------>paper_class3=2 ------>paper_class2=1 ------>vol=34 ------>confirm_bywho=chshih43 ------>insert_bywho=shang-jyh ------>Jurnal_Rank=11.8 ------>authors4_c=??? ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=764 ------>medlineContent= ------>unit=E0400 ------>insert_date=20060502 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c=??? ------>score=500 ------>journal_name=Critical Care Medicine ------>paper_name=Insulin attenuates endotoxin-induced acute lung injury in conscious rats ------>confirm_date=20081208 ------>tch_id=092081 ------>pmid=16505662 ------>page1=758 ------>fullAbstract=OBJECTIVES: To investigate the effects of insulin on the acute lung injury induced by lipopolysaccharide using a conscious rat model. DESIGN: Prospective, randomized, controlled animal study. SETTING: University research laboratory. SUBJECTS: A total of 190 adult male Sprague-Dawley rats weighing 250-300 g. INTERVENTIONS: Endotoxemia was induced by intravenous infusion of lipopolysaccharide. Lipopolysaccharide at various doses (0, 1, 5, 10, 20, and 30 mg/kg, n=10 for each dose) was administered intravenously in 20 mins. Insulin infusion at doses of 0.5, 1, and 5 microU/kg/min was given 5 mins before lipopolysaccharide administration. Plasma glucose was clamped at 90-110 mg/dL by infusion of 10-80% glucose solution. Insulin and glucose infusion (0.01 mL/min) was started 5 mins before lipopolysaccharide and continued for 120 mins. The rats received a total of 60, 120, and 600 microU/kg insulin as well as 0.12, 0.36, and 0.96 g of glucose in respective groups. The animals were then observed for 4 hrs. MEASUREMENTS AND MAIN RESULTS: The extent of acute lung injury was evaluated by lung weight/body weight ratio, lung weight gain, protein concentration in bronchoalveolar lavage, and exhaled nitric oxide. We also measured plasma nitrate/nitrite and methyl guanidine. In addition, histopathologic changes of the lung were examined. Lipopolysaccharide caused systemic hypotension and severe acute lung injury with increases in plasma nitrate/nitrite and methyl guanidine. Pretreatment with insulin infusion at doses of 0.5, 1, and 5 microU/kg/min mitigated or prevented systemic hypotension and the development of acute lung injury, depending on the dose. Insulin also attenuated the lipopolysaccharide-induced increases in nitrate/nitrite and methyl guanidine. CONCLUSIONS: Insulin is effective in reducing or preventing the lipopolysaccharide-induced increases in plasma nitrate/nitrite and methyl guanidine and the occurrence of acute lung injury. ------>tmu_sno=None ------>sno=13569 ------>authors2=Yeh DY ------>authors3=Liou HL ------>authors4=Kao SJ ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Chen HI ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=0 ------>updateTitle=Insulin attenuates endotoxin-induced acute lung injury in conscious rats. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=1 |