| Wu CC |
------>authors3_c=??? ------>paper_class1=2 ------>Impact_Factor=None ------>paper_class3=0 ------>paper_class2=0 ------>vol= ------>confirm_bywho=lm ------>insert_bywho=ccwu ------>Jurnal_Rank=None ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=24 ------>paper_class2Letter=None ------>page2=240 ------>medlineContent= ------>unit=E0117 ------>insert_date=20060502 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c=??? ------>score=41 ------>journal_name=????????91???13??1???????24?????? ------>paper_name=?????????CFTR???????????? ------>confirm_date=20060502 ------>tch_id=077009 ------>pmid=19914879 ------>page1=240 ------>fullAbstract=BACKGROUND: The pathogenesis of infection with Burkholderia cepacia complex (Bcc) organisms may be linked to its capacity to invade respiratory epithelium. METHODS: An antibiotic exclusion assay was used to study B. dolosa AU4459 and B. cenocepacia J2315 invasion into wild-type (WT) and CFTR-deficient respiratory epithelial cells. Inhibitors were used to evaluate Bcc invasion dependency on host microtubule (mt) and microfilament (mf) systems. RESULTS: B. dolosa entered WT-CFTR cells with 5-fold greater efficiency than CFTR deficient cells (25% vs 5%, respectively). Invasion dropped to <0.5% after either mf or mt inhibition. B. cenocepacia entered WT (0.05%) and CFTR-deficient cells (0.07%) with similarly low efficiencies, which significantly decreased with either mf or mt inhibition (0.008% and 0.002%, respectively). CONCLUSION: B. dolosa and B. cenocepacia enter respiratory epithelial cells in a mf and mt dependent fashion. Mutated CFTR leads to less internalization of B. dolosa, but not B. cenocepacia. ------>tmu_sno=None ------>sno=13588 ------>authors2=Chiang HS ------>authors3=Lin YM ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Wu CC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=1 ------>updateTitle=Entry of Burkholderia organisms into respiratory epithelium: CFTR, microfilament and microtubule dependence. ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2002 ------>submit_flag=None ------>publish_month=8 |