| Shieh YH |
------>authors3_c= ------>paper_class1=2 ------>Impact_Factor=None ------>paper_class3=0 ------>paper_class2=0 ------>vol= ------>confirm_bywho=shiemin ------>insert_bywho=syh001 ------>Jurnal_Rank=None ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=17 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0100 ------>insert_date=20060503 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=-47 ------>journal_name=????????/???????? ------>paper_name=??????65???????????????? ------>confirm_date=20071127 ------>tch_id=079010 ------>pmid=19921562 ------>page1= ------>fullAbstract=OBJECTIVE: To investigate the effects of duodental-jejunal bypasss(DJB) and sleeve gastrectomy(SG) on the expression of liver glucokinase(GCK) in diabetic rats. METHODS: Animal models of Goto-Kakizaki rats and Sprague-Dawley rats were established by DJB and SG. Results of fasting glycemia and insulin were compared. Liver tissue was harvested 8 weeks postoperatively.Quantitative real-time PCR and Western blot were used to detect liver GCK mRNA and protein expression after operation. RESULTS: Fasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined 3 day and 1, 2, 4, 6, 8 weeks postoperatively(all P <0.01), while Sham group only droped 3 day and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively(P >0.05). Fasting plasma glucose levels of each group in SD rats did not change after operation. In GK rats, GCK mRNA level (1.45 +/-0.29) and protein expression (494.25 +/-30.25) after DJB were higher than Sham group (1.05 +/-0.19 and 409.13 +/-26.86) and control group (1.04 +/-0.17 and 404.75 +/-30.90). GCK mRNA level and protein expression after SG were 0.65 +/-0.25 and 345.25 +/-28.13 respectively, which were significantly lower than those in control group(all P <0.01). All the groups in SD rats experienced similar GCK expression change. CONCLUSION: Both DJB and SG can decrease the plasma glucose levels of GK rats, while there are different effects on the expression of liver GCK. ------>tmu_sno=None ------>sno=13637 ------>authors2= ------>authors3= ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Shieh YH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=2 ------>updateTitle=[Effects of duodental-jejunal bypasss and sleeve gastrectomy on the expression of liver glucokinase in diabetic rats.] ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=4 |