| Tai-Chih Kuo |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.904 ------>paper_class3=2 ------>paper_class2=1 ------>vol=273 ------>confirm_bywho=None ------>insert_bywho=tckuo ------>Jurnal_Rank=35.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=15 ------>paper_class2Letter=None ------>page2=971 ------>medlineContent= ------>unit=000 ------>insert_date=20060504 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=1 ------>ISSN= ------>authors_c=??? ------>score=500 ------>journal_name=Biochemicl Biophysical Research Communication ------>paper_name=A Kinetically Efficient Form of the Chlamydomonas Self-Splicing Ribosomal RNA Precursor ------>confirm_date=None ------>tch_id=094060 ------>pmid=10891356 ------>page1=967 ------>fullAbstract=The 23S rRNA gene of Chlamydomonas reinhardtii contains a group IA3 intron, Cr.LSU, whose splicing is essential for cell growth. To better understand Cr.LSU splicing, kinetic analyses were undertaken with 23S.3, a preRNA previously shown to self-splice. Self-splicing of 23S.3 showed biphasic kinetics, with only approximately 33% reacting efficiently. Removal of a region of the 5~ exon that could potentially interfere with the intron core (i.e., P3) increased the size (53%) of the active fraction. Replacement of the large P6a-extension by a 20-nt stem-loop further increased the active fraction to approximately 80%. The k(cat) and K(G)(M) for self-splicing (first step) by these latter RNAs were approximately 1 min(-1) and approximately 20 microM, respectively. These results indicate that Cr.LSU is a highly efficient ribozyme whose folding in vitro is impeded by exonic and/or intronic sequences. The implications for in vivo splicing of Cr.LSU are discussed. ------>tmu_sno=None ------>sno=13708 ------>authors2=David L Herrin ------>authors3= ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Tai-Chih Kuo ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=David L Herrin ------>publish_area=0 ------>updateTitle=A kinetically efficient form of the Chlamydomonas self-splicing ribosomal RNA precursor. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2000 ------>submit_flag=None ------>publish_month=5 |