| Yang KC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=1 ------>paper_class2=1 ------>vol=11 ------>confirm_bywho=yangyuan ------>insert_bywho=ycliang ------>Jurnal_Rank=None ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=3045 ------>medlineContent= ------>unit=E0300 ------>insert_date=20060505 ------>iam=4 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=375 ------>journal_name=World J Gastroenterol. ------>paper_name=Molecular mechanisms of denbinobin-induced anti-tumorigenesis effect in colon cancer cells. 11(20): 3040-3045. ------>confirm_date=20060505 ------>tch_id=089135 ------>pmid=15918186 ------>page1=3040 ------>fullAbstract=AIM: To explore both the in vitro and in vivo effects of denbinobin against colon cancer cells and clarify its underlying signal pathways. METHODS: We used COLO 205 cancer cell lines and nude mice xenograft model to study the in vitro and in vivo anti-cancer effects of denbinobin. RESULTS: Denbinobin at concentration of 10-20 micromol/L dose-dependently suppressed COLO 205 cell proliferation by MTT test. Flow cytometry analysis and DNA fragmentation assay revealed that 10-20 micromol/L denbinobin treatment induced COLO 205 cells apoptosis. Western blot analysis showed that caspases 3, 8, 9 and Bid protein were activated by denbinobin treatment to COLO 205 cells accompanied with cytochrome c and apoptosis-inducing factor (AIF) translocation. Pretreatment of MEK 1 inhibitor (U10126), but not p38 inhibitor (SB203580) and JNK inhibitor (SP600125), reversed denbinobin-induced caspase 8, 9 and Bid activation in COLO 205 cells suggesting that extracellular signal-regulated kinase were involved in the denbinobin-induced apoptosis in COLO 205 cells. Significant regression of tumor up to 68% was further demonstrated in vivo by treating nude mice bearing COLO 205 tumor xenografts with denbinobin 50 mg/kg intraperitoneally. CONCLUSION: Our findings suggest that denbinobin could inhibit colon cancer growth both in vitro and in vivo. Activation of extrinsic and intrinsic apoptotic pathways and AIF were involved in the denbinobin-induced COLO 205 cell apoptosis. ------>tmu_sno=None ------>sno=13781 ------>authors2=Uen YH ------>authors3=Suk FM ------>authors4=Liang YC ------>authors5=Wang YJ ------>authors6=Ho YS, Li IH, Lin SY. ------>authors6_c= ------>authors=Yang KC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Molecular mechanisms of denbinobin-induced anti-tumorigenesis effect in colon cancer cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=20 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=10 |