Chen CJ |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.518 ------>paper_class3=2 ------>paper_class2=1 ------>vol=9 ------>confirm_bywho=kyhsu ------>insert_bywho=thwu ------>Jurnal_Rank=7.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=784 ------>medlineContent= ------>unit=G0100 ------>insert_date=20061016 ------>iam=5 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Combinatorial Chemistry & High Throughput Screening ------>paper_name=A Screening Platform for Compounds with Potential Immuno-Regulatory Activities Using Human Cord Blood Mononuclear Cells. ------>confirm_date=20061201 ------>tch_id=091080 ------>pmid=17168683 ------>page1=777 ------>fullAbstract=A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and gamma-dodecalactone (gamma-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3(+) T cells, CD56(+) NK cells and CD1a(+) dendritic cells apparently increased after being treated with gamma-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following gamma-DDL treatment. These results demonstrated that the gamma-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds. ------>tmu_sno=None ------>sno=14148 ------>authors2=Tsai CC ------>authors3=Hsieh JF ------>authors4=Chien CM ------>authors5=Wu TH ------>authors6=Chen ST ------>authors6_c= ------>authors=Chen CJ ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=A screening platform for compounds with potential immuno-regulatory activities using human cord blood mononuclear cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=10 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=12 |