| Chuang CC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.108 ------>paper_class3=2 ------>paper_class2=1 ------>vol=115 ------>confirm_bywho=tedfan ------>insert_bywho=tedfan ------>Jurnal_Rank=73.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=232 ------>medlineContent= ------>unit=E0101 ------>insert_date=20061213 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=-24 ------>journal_name=Experimental Parasitology ------>paper_name=Angiostrongylus cantonensis: Apoptosis of in inflammatory cells induced by treatment with mebendazole or/and interleukin 12 in mice ------>confirm_date=20070803 ------>tch_id=084017 ------>pmid=19885559 ------>page1=226 ------>fullAbstract=Interleukin-12 has been elucidated as a powerful anti-cancer factor in pre-clinical research. However, the obstacles of this modality that emerged from human clinical trails included the toxicity of repeated large dose administration and short effective duration. Therefore, a prolonged, constant therapeutic level of interleukin-12 is required to reduce the adverse effects and enhance the therapeutic efficacy. In this study, 54 nude mice were divided into three groups treated with rAAV2 encoding interleukin-12, rAAV2 vector, and PBS, respectively. All nude mice received human glioblastoma multiforme cell line DBTRG implantation. The biochemistry studies included serum levels of interleukin-12, isotypes of immunoglobulin, interferon-gamma, and TNF-alpha. The activated NK cells were sorted from the spleen by flow cytometry and the cytotoxicity of NK cells were evaluated by LDH assay. In the rAAV2 encoding interleukin-12 group, substantial expression of interleukin-12 was obtained with a serum level of 120-150 pg/ml through the experimental course and a significant increase of activated NK cells was achieved. The splenocytes extracted from the spleen in rAAV2 encoding IL-12 mice strongly exhibited cytotoxic activity compared to the control groups (p<0.001). The IgG1, IgG2a, and IgM also showed a significant increase in the rAAV2 encoding IL-12 group compared to the control groups (p<0.05). The tumor growth rate decreased obviously in the rAAV2 encoding IL-12 group with a significant difference from the control groups (p<0.001). This study demonstrated an encouraging result of immunomodulative therapy in malignant brain tumors by rAAV2 carrying IL-12 through activating NK cells. ------>tmu_sno=None ------>sno=14373 ------>authors2=Chen CW ------>authors3=Fan CK ------>authors4=Su KE ------>authors5=Tsai YT ------>authors6=Du WY ------>authors6_c= ------>authors=Chuang CC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=AAV2-mediated interleukin-12 in the treatment of malignant brain tumors through activation of NK cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=3 |