Au HK |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.114 ------>paper_class3=2 ------>paper_class2=1 ------>vol=84 ------>confirm_bywho=leehorng ------>insert_bywho=kaosh ------>Jurnal_Rank=10.5 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1108 ------>medlineContent= ------>unit=E0310 ------>insert_date=20061215 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Fert Steril ------>paper_name=Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells. ------>confirm_date=20070507 ------>tch_id=089113 ------>pmid=16210000 ------>page1=1104 ------>fullAbstract=OBJECTIVE: To evaluate the transcription and translation ability of mitochondria in terminally differentiated granulosa cells, these cells were incubated with ionic calcium. DESIGN: Prospective laboratory research. SETTING: In vitro fertilization laboratory in a university hospital. PATIENT(S): Granulosa cells were harvested from 50 female patients undergoing IVF. INTERVENTION(S): Analysis of mitochondrial gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and of mitochondrial-encoded proteins by Western blot. MAIN OUTCOME MEASURE(S): Comparison of the RNA expression levels of genes including cytochrome c oxidase subunit I (COX I), adenosine triphosphate synthase 6 (ATPase 6), flavoprotein, and succinate-ubiquinone oxidoreductase, and protein levels of COX I and flavoprotein in different calcium ion treatment groups. RESULT(S): There were dose-dependent increases in RNA expressions of the four genes analyzed from granulosa cells cultured in a serial concentration of calcium ions. This effect was abolished when cells were preincubated with the extracellular calcium-chelating agent, Ethylene glycol-bis (2-aminoethylether)-N,N,N~,N~-tetraacetic acid (EGTA). The effect of ionic calcium on both the nuclear- and mitochondrial-encoded subunits also was determined. Expression levels of mitochondrial transcription factor A in RNA significantly increased in granulosa cells that were exposed for 24 and 48 hours to 0.5 and 1 microM A23187. CONCLUSION(S): The present study is the first report to present calcium-dependent increases in the transcription and translation levels of both nuclear-encoded and mitochondrial-encoded mitochondrial respiratory enzyme subunits and also indicates that mitochondrial transcription factor A is involved in mitochondrial biogenesis. ------>tmu_sno=None ------>sno=14413 ------>authors2=Yeh TS ------>authors3=Kao SH ------>authors4=Shih CM ------>authors5=Hsieh RH ------>authors6=Tzeng CR ------>authors6_c= ------>authors=Au HK ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Calcium-dependent up-regulation of mitochondrial electron transfer chain gene expressions in human luteinized granulosa cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=Suppl2 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=1 |