| Chang, Y. |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.123 ------>paper_class3=2 ------>paper_class2=1 ------>vol= ------>confirm_bywho=chlin ------>insert_bywho=sheujr ------>Jurnal_Rank=41.6 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0200 ------>insert_date=20061225 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=384 ------>journal_name=Acta Pharmacol. Sinica ------>paper_name=Tetramethylpyrazine suppresse4s the HIF-1?, TNF-?, and active caspase-3 expression in middle cerebral artery occlusion-induced brain ischemia in rats. ------>confirm_date=20061226 ------>tch_id=082005 ------>pmid=17302993 ------>page1= ------>fullAbstract=AIM: To examine the detailed mechanisms underlying the inhibitory effect of tetramethylpyrazine (TMPZ) in inflammatory and apoptotic responses induced by middle cerebral artery occlusion (MCAO) in rats. METHODS: MCAO-induced focal cerebral ischemia in rats was used in this study. The hypoxia-inducible factor-1alpha(HIF-1alpha), activation of caspase-3, and TNF-alpha mRNA transcription in ischemic regions were detected by immunoblotting and RT-PCR, respectively. Anti-oxidative activity was investigated using a thiobarbituric acid-reactive substance (TBARS) test in rat brain homogenate preparations. RESULTS: We showed the statistical results of the infarct areas of solvent- and TMPZ (20 mg/kg)-treated groups at various distances from the frontal pole in MCAO-induced focal cerebral ischemia in rats. Treatment with TMPZ (20 mg/kg) markedly reduced the infarct area in all regions, especially in the third to fifth sections. MCAO-induced focal cerebral ischemia was associated with increases in HIF-1alpha and the activation of caspase-3, as well as TNF-alpha transcription in ischemic regions. These expressions were markedly inhibited by treatment with TMPZ (20 mg/kg). However, TMPZ (0.5-5 mmol/L) did not significantly inhibit TBARS reaction in rat brain homogenates. CONCLUSION: The neuroprotective effect of TMPZ may be mediated at least by a portion of the inhibition of HIF-1alpha and TNF-alpha activations, followed by the inhibition of apoptosis formation (active caspase-3), resulting in a reduction in the infarct volume in ischemia-reperfusion brain injury. Thus, TMPZ treatment may represent an ideal approach to lowering the risk of or improving function in ischemia- reperfusion brain injury-related disorders. ------>tmu_sno=None ------>sno=14507 ------>authors2=Hsiao, G. ------>authors3=Chen, S.H. ------>authors4=Chen, Y.C. ------>authors5=Lin, J.H. ------>authors6=Lin, K.H., Chou, D.S., Sheu, J.R. ------>authors6_c= ------>authors=Chang, Y. ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Tetramethylpyrazine suppresses HIF-1alpha, TNF-alpha, and activated caspase-3 expression in middle cerebral artery occlusion-induced brain ischemia in rats. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=1 |