| Wu, C.J. |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.956 ------>paper_class3=2 ------>paper_class2=1 ------>vol=132 ------>confirm_bywho=chlin ------>insert_bywho=sheujr ------>Jurnal_Rank=21.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=111 ------>medlineContent= ------>unit=E0200 ------>insert_date=20061225 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=J. Surg. Res. ------>paper_name=Modulation of monocyte-derived dendritic cell differentiation is associated with Ischemic acute renal failure. ------>confirm_date=20061226 ------>tch_id=082005 ------>pmid=16330051 ------>page1=104 ------>fullAbstract=BACKGROUND: Dendritic cells (DCs) play a central role in both stimulating and suppressing immune responses and are impacted by surgical injury, exercise, and other physiological stressors. This study aims to determine whether renal ischemia/reperfusion (I/R) injury alters the differentiation, maturation, and activation of DCs from peripheral blood monocytes (PBMo). MATERIALS AND METHODS: Sprague-Dawley (SD) rats were subjected to I/R injury or sham-operated. Creatinine clearance (CCr) was monitored daily during the 14 days of reperfusion that followed the ischemic insult. At 2 and 14 days of reperfusion, the following properties of PBMo derived-DCs were assessed: the amount of generated DCs, surface markers [CD11c, CD80, CD86, and MHC-II (IA)], and functional status including magnitude of mixed lymphocyte reaction (MLR), production of IL-12 p70 by DCs, and production of IFN-gamma and IL-4 by DC-stimulated T cells. RESULTS: CCr was greatly reduced in the injured rats 0 to 4 days after ischemia. Two days after I/R injury to kidney, the numbers of DCs differentiated from PBMo, IL-12 production by DCs, expression of MHC-II (IA), and IFN-gamma production by DC-stimulated T cells were significantly increased in the I/R injured group (compared to the sham-operated group). After 14 days of reperfusion, there was no between-group differences in the numbers of DCs derived from PBMo, MLR, expression of CD80, CD86, and MHC-II (IA), and production of IL-12, IFN-gamma, and IL-4. CONCLUSIONS: The increases seen at 2 days of reperfusion may reflect a preparatory step in the renal I/R injury pathway. The relationship between up-regulation of DC differentiation and ischemic acute renal failure (ARF) remains to be elucidated. ------>tmu_sno=None ------>sno=14510 ------>authors2=Sheu, J.R. ------>authors3=Chen, H.H. ------>authors4=Liao, H.F. ------>authors5=Yang, Y.C. ------>authors6=Yang, S., Chen, Y.J. ------>authors6_c= ------>authors=Wu, C.J. ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Modulation of monocyte-derived dendritic cell differentiation is associated with ischemic acute renal failure. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=1 |