| Chao YC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.690 ------>paper_class3=2 ------>paper_class2=1 ------>vol=118 ------>confirm_bywho=amel ------>insert_bywho=jhorng ------>Jurnal_Rank=11.0 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=117 ------>medlineContent= ------>unit=G0100 ------>insert_date=20070412 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Journal of Controlled Release ------>paper_name=Ethanol enhanced in vivo gene delivery with non-ionic polymeric micelles inhalation ------>confirm_date=20071129 ------>tch_id=082003 ------>pmid=17258837 ------>page1=105 ------>fullAbstract=Modifications of both carriers and host barriers have been investigated for efficient inhalation gene delivery to lung. Here we used a biocompatible, non-ionic poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) polymeric micelles (PM) as a carrier and combined it with ethanol to enhance membrane penetration of delivered DNA. The inhalation delivery with six 100 microg doses of pCMV-Lac Z with PM co-formulated with 10%-40% ethanol to nude mice in 2 days at 8 h interval was performed. The beta-galatosidase (beta-Gal) activity was assessed using chlorophenol red-beta-d galactopyranoside (CPRG) and X-gal staining for quantitative and qualitative analysis in tissues. The results showed that beta-Gal activity was significantly increased by 38% in lung around bronchioles when inhalation with PM and 10% ethanol was given. The 10% ethanol also increased the intracellular apparent permeability by 42% in stomach and by 141% in intestine at 48 h after the first dosage of delivery. Also delivery of DNA encoding a functional human cystic fibrosis transmembrane protein (CFTR) using the same inhalation delivery method co-formulated with 10% ethanol, an increased expression of CFTR in lung was detected by immunostaining. We concluded that 10% ethanol co-formulated with the PM system could enhance inhaled gene delivery to airway and gastrointestinal (GI) tract. ------>tmu_sno=None ------>sno=14841 ------>authors2=Chang SF ------>authors3=Lu SC ------>authors4=Hwang TC ------>authors5=Hsieh WH ------>authors6=Liaw J ------>authors6_c= ------>authors=Chao YC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Ethanol enhanced in vivo gene delivery with non-ionic polymeric micelles inhalation. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=3 |