Shyu KG |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=4.148 ------>paper_class3=2 ------>paper_class2=1 ------>vol=313 ------>confirm_bywho=chlin ------>insert_bywho=shyukg ------>Jurnal_Rank=25.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1191 ------>medlineContent= ------>unit=E0200 ------>insert_date=20070412 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Exp Cell Res ------>paper_name=Hypoxia-inducible factor 1alpha regulates lung adenocarcinoma cell invasion ------>confirm_date=20070413 ------>tch_id=092067 ------>pmid=17335808 ------>page1=1181 ------>fullAbstract=We studied the role of hypoxia-inducible factor-1alpha (HIF-1alpha) in human lung adenocarcinoma cell invasion using a metastatic cell model composed of low invasive CL1 and highly invasive CL1-5 cells. We showed that HIF-1alpha was expressed in CL1-5 but not in CL1 cells under normoxic condition, and that inhibition of HIF-1alpha expression by a small interfering RNA decreased invasiveness of CL1-5 cells. Complementary, overexpression of HIF-1alpha increased the invasiveness of CL1 and gastric cancer SC-M1 cells. Subsequently, we showed that urokinase-type plasminogen activator receptor (uPAR), and matrix metalloproteinases (MMPs) 1 and 2 were critical in HIF-1alpha-induced invasion. Mechanistic studies revealed that HIF-1alpha overexpression could increase the expression of uPAR and MMP1, but not MMP2. However, ELISA assays on the conditioned media generated from control CL1 and CL1 cells overexpressing HIF-1alpha showed that overexpression of HIF-1alpha increased the levels of endogenous free active MMP2 and total free MMP2, and the former was blocked by inhibition of MMP1 expression. We conclude that (i) HIF-1alpha overexpression enhances lung cancer cell invasion at least through up-regulating the expression and activities of uPAR, MMP1, and MMP2; and (ii) induction of MMP1 participates in cell invasion and also plays an important role in HIF-1alpha-induced activation of MMP2. ------>tmu_sno=None ------>sno=14886 ------>authors2=Hsu FL ------>authors3=Wang MJ ------>authors4=Wang BW ------>authors5=Lin S ------>authors6= ------>authors6_c= ------>authors=Shyu KG ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Hypoxia-inducible factor 1alpha regulates lung adenocarcinoma cell invasion. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=4 |