| Lin JC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.047 ------>paper_class3=2 ------>paper_class2=1 ------>vol=45 ------>confirm_bywho=hj2166 ------>insert_bywho=chwu ------>Jurnal_Rank=9.7 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=944 ------>medlineContent= ------>unit=E0110 ------>insert_date=20070418 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Food and Chemical Toxicology ------>paper_name=Induction of apoptosis and cell-cycle arrest in human colon cancer cells by meclizine ------>confirm_date=20070418 ------>tch_id=071004 ------>pmid=17222494 ------>page1=935 ------>fullAbstract=Meclizine (MEC), a histamine H1 antagonist, is used for the treatment of motion sickness and vertigo. In this study, we demonstrate that MEC dose-dependently induced apoptosis in human colon cancer cell lines (COLO 205 and HT 29 cells). Results of a DNA ladder assay revealed that DNA ladders appeared with MEC treatment in COLO 205 cells at dosage of >50 microM. In addition, the total cell number decreased dose-dependently after treatment with MEC in COLO 205 and HT 29 cells. Using flow cytometry, the percentage of COLO 205 cells arrested at G0/G1 phase increased dose-dependently. Analysis of changes in cell-cycle arrest-associated proteins with Western blotting showed that p53 and p21 were upregulated after treatment with MEC. The kinase activities of cyclin-dependent kinase 2 (CDK2) and CDK4 were suppressed in MEC-treated cells. As for apoptosis, MEC may induce upregulation of p53 and downregulation of Bcl-2, thus causing the release of cytochrome C from mitochondria and the translocation of apoptosis-inducing factor (AIF) to the nucleus. This resulted in the activation of caspase 3, 8, and 9. Our results provide the molecular basis of MEC-induced apoptosis and cell-cycle arrest in human colon cancer cells. ------>tmu_sno=None ------>sno=15064 ------>authors2=Ho YS ------>authors3=Lee JJ ------>authors4=Liu CL ------>authors5=Yang TL ------>authors6=Wu CH ------>authors6_c= ------>authors=Lin JC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Induction of apoptosis and cell-cycle arrest in human colon cancer cells by meclizine. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=1 |