| Chou YH |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.047 ------>paper_class3=2 ------>paper_class2=1 ------>vol= ------>confirm_bywho=hj2166 ------>insert_bywho=chwu ------>Jurnal_Rank=9.7 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0110 ------>insert_date=20070419 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Food and Chemical Toxicology ------>paper_name=Tubulozole-induced G2/M cell cycle arrest in human colon cancer cells through formation of microtubule polymerization mediated by ERK1/2 and Chk1 kinase activation ------>confirm_date=20081202 ------>tch_id=071004 ------>pmid=17329004 ------>page1= ------>fullAbstract=Our studies demonstrated that human colon cancer cells (COLO 205), with higher expression level of check point kinase 1 (Chk1), were more sensitive to microtubule damage agent Tubulozole (TUBU) induced G2/M phase arrest than normal human colon epithelial (CRL) cells. TUBU (10 microM, for 3h) treatment resulted in rapid and sustained phosphorylation of Cdc25C (Ser-216) leading to increased 14-3-3beta binding. This resulted in increased nuclear translocation. In addition, TUBU induced phosphorylation of the Cdc25C (Ser-216) and Bad (Ser-155) proteins were blocked by Chk1 SiRNA-transfection. Surprisingly, cellular apotosis was observed in cells treated with TUBU after Chk1 SiRNA inhibition. We further demonstrated that extracellular signal-regulated kinase (ERK) activation by TUBU was needed for Chk1 kinase activation and microtubule formation as shown by the attenuation of these responses by the ERK1/2 specific inhibitor PD98059. However, TUBU induced ERK1/2 phosphorylation was not blocked in the Chk1 SiRNA-transfected COLO 205 cells. These results imply that ERK1/2 mediated Chk1 activation may be play an important role in determining TUBU induced G2/M arrest or apoptosis in COLO 205 cells. ------>tmu_sno=None ------>sno=15111 ------>authors2=Ho YS ------>authors3=Wu CC ------>authors4=Chai CY ------>authors5=Chen SC ------>authors6=Lee CH, Tsai PS, Wu CH ------>authors6_c= ------>authors=Chou YH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Tubulozole-induced G2/M cell cycle arrest in human colon cancer cells through formation of microtubule polymerization mediated by ERK1/2 and Chk1 kinase activation. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=1 |