Liu SY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=0.474 ------>paper_class3=2 ------>paper_class2=1 ------>vol=104 ------>confirm_bywho=None ------>insert_bywho=syliu ------>Jurnal_Rank=76.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=119 ------>medlineContent= ------>unit=000 ------>insert_date=20070426 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=1 ------>ISSN=0929-6646 ------>authors_c= ------>score=500 ------>journal_name=Journal of the Formosan Medical Association ------>paper_name=Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9. ------>confirm_date=None ------>tch_id=078008 ------>pmid=15765166 ------>page1=113 ------>fullAbstract=BACKGROUND AND PURPOSE: The effects of areca quid (AQ) consumption on salivary matrix metalloproteinases (MMPs) which may participate in tumor invasion and metastasis remains unclear. This study assessed the change in salivary MMP-9 protein levels 2 hours after 5-minute AQ chewing stimulation (AQCS) in non-AQ users and the expression profile of this proteinase in saliva and tumor specimens of oral squamous cell carcinoma (OSCC) patients with a history of AQ use. METHODS: MMP-9 transcript was analyzed by reverse transcription-polymerase chain reaction. MMP-9 protein level was measured by both Western blot and gelatin zymography. RESULTS: The protein level of salivary MMP-9 was 3.1- to 8.9-fold enhanced 2 h after AQCS in 3 healthy volunteers as revealed by Western blot and zymography. As a control, gum chewing did not significantly change salivary MMP-9 protein level. Expression of MMP-9 transcript was found in 25 of 28 OSCC specimens and significantly correlated with cervical lymph node metastasis (p = 0.037). All of the 8 tested OSCC tissue homogenate samples available and all 12 saliva samples from 12 oral tumor outpatients were positive for MMP-9 protein. CONCLUSIONS: Elevation of MMP-9 may be one of the net effects of AQCS in vivo, which may play a role in the pathogenesis of oral mucosal lesions. Furthermore, the association of MMP-9 expression with neck-lymph-node metastasis may imply a significant role of MMP-9 in the progression of OSCC among patients with a history of AQ use in Taiwan. ------>tmu_sno=None ------>sno=15172 ------>authors2=Lin MH ------>authors3=Yang SC ------>authors4=Yen CY ------>authors5=Chiang WF ------>authors6=Kuo YY,Lee CH,Liu CY ------>authors6_c= ------>authors=Liu SY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Areca quid chewing enhances the expression of salivary matrix metalloproteinase-9. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=2 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=2 |