| Liu SY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=0.474 ------>paper_class3=2 ------>paper_class2=1 ------>vol=104 ------>confirm_bywho=seanlee ------>insert_bywho=syliu ------>Jurnal_Rank=76.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=397 ------>medlineContent= ------>unit=F0100 ------>insert_date=20070426 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=10103287 ------>authors_c= ------>score=500 ------>journal_name=Journal of the Formosan Medical Association ------>paper_name=Increased expression of matrix metalloproteinase-2 in oral cells after short-term stimulation and long-term usage of areca quid ------>confirm_date=20070501 ------>tch_id=078008 ------>pmid=16037826 ------>page1=390 ------>fullAbstract=BACKGROUND AND PURPOSE: Arecoline, an areca quid (AQ) component, has been shown to inhibit the secretion and activity of matrix metalloproteinase-2 (MMP-2) in fibroblast cultures. This study assessed whether MMP-2 expression was inhibited in the saliva samples and tumor specimens of oral tumor patients with a long-term history of AQ consumption. The net effect of crude AQ extract (AQE) on MMP-2 expression by oral cells was also investigated. METHODS: Western blot analysis, zymography, and reverse transcriptase-polymerase chain reaction were used to detect MMP-2 protein and mRNA in saliva and tumor samples, as well as in the conditioned media (CM) of oral cell cultures. RESULTS: The level of MMP-2 protein was significantly higher in the saliva samples of 12 oral tumor patients who had a minimum 10-year AQ-consuming history than in those of 12 non-AQ-using healthy controls (p < 0.05). MMP-2 mRNA was expressed in 26 of 28 oral squamous cell carcinoma (OSCC) specimens. MMP-2 protein was also detectable in the tested OSCC homogenates. Short-term stimulation with 10% AQE increased the secretion of MMP-2 protein in the CM of oral epidermoid carcinoma cell Meng-1 (an OSCC cell line) and oral fibroblasts. CONCLUSIONS: MMP-2 expression is elevated rather than inhibited in most oral tumor patients with long-term AQ usage. Short-term AQE stimulation also increases the secretion of MMP-2 by oral epithelial cells and fibroblasts. Our results suggest that AQ consumption may promote oral tumor progression through the induction of MMP-2 secretion. ------>tmu_sno=None ------>sno=15177 ------>authors2=Lin MH ------>authors3=Yang CY ------>authors4=Huang GC ------>authors5=Yen YC ------>authors6=Chiang WF,KuoYY.Chen LL,Lee CH,Liu YC ------>authors6_c= ------>authors=Liu SY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Increased expression of matrix metalloproteinase-2 in oral cells after short-term stimulation and long-term usage of areca quid. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=6 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=6 |