| Liu SY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.266 ------>paper_class3=2 ------>paper_class2=1 ------>vol=43 ------>confirm_bywho=seanlee ------>insert_bywho=syliu ------>Jurnal_Rank=8.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1033 ------>medlineContent= ------>unit=F0100 ------>insert_date=20070426 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=1368-8375 ------>authors_c= ------>score=500 ------>journal_name=Oral Oncology ------>paper_name=Up-regulation of matrix metalloproteinase-8 by betel quid extract and arecoline and its role in 2D motility. ------>confirm_date=20080428 ------>tch_id=078008 ------>pmid=17306610 ------>page1=1026 ------>fullAbstract=Betel quid (BQ) and matrix metalloproteinase-8 (MMP-8) play roles in oral diseases. Here, we analyzed the regulation of MMP-8 by BQ and its effect on cell migration. We found that BQ extract (BQE) increased the secretion of an 85kDa caseinolytic proteinase, specifically precipitated by an anti-MMP-8 antibody, in the culture medium of OECM-1, an oral squamous cell carcinoma (OSCC) cell line. BQE also stimulated MMP-8 secretion in an esophageal carcinoma cell line, CE81T/VGH, in a dose-dependent manner, and MMP-8 protein was maximally expressed at 24h after BQE treatment in OECM-1. The BQE-induced MMP-8 expression was dose-dependently inhibited by PD98059. Arecoline, the major alkaloid of areca nut, was tested to dose-dependently up-regulate MMP-8 protein level. Moreover, both arecoline- (4.7-fold) and BQE-selected (5.5-fold) CE81T/VGH cells expressed higher MMP-8 protein level and exhibited enhanced two-dimensional (2D) motility (p=0.009 in both cells) than parental cells. The enhanced motility of arecoline- (p=0.006) and BQE-selected (p=0.002) cells was both specifically blocked by an anti-MMP-8 antibody. We conclude that BQ may accelerate tumor migration by stimulating MMP-8 expression through MEK pathway in at least some carcinomas of the upper aerodigestive tract. Furthermore, arecoline may be one of the positive MMP-8 regulators among BQ ingredients. ------>tmu_sno=None ------>sno=15186 ------>authors2=Liu YC ------>authors3=Lin YU ------>authors4=Huang WT ------>authors5=Huanf GC ------>authors6=Chen TC,Lin MH ------>authors6_c= ------>authors=Liu SY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Up-regulation of matrix metalloproteinase-8 by betel quid extract and arecoline and its role in 2D motility. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=10 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=1 |