Liu SY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.661 ------>paper_class3=2 ------>paper_class2=1 ------>vol=36 ------>confirm_bywho=seanlee ------>insert_bywho=syliu ------>Jurnal_Rank=36.7 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=435 ------>medlineContent= ------>unit=F0100 ------>insert_date=20070426 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=0904-2512 ------>authors_c= ------>score=500 ------>journal_name=Journal of Oral Pathology & Medicine ------>paper_name=Requirement of MMP-3 in anchorage-independent growth of oral squamous cell carcinomas ------>confirm_date=20080428 ------>tch_id=078008 ------>pmid=17617837 ------>page1=430 ------>fullAbstract=BACKGROUND: Matrix metalloproteinase-3 (MMP-3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs). MATERIALS AND METHODS: Specimens of seven oral pre-malignant lesions (OPMLs) and 92 OSCCs were subjected to MMP-3 detection by RT-PCR and Western blot. Antisense oligodeoxynucleotides (AODNs) of MMP-3 were used to transfect OSCC (OECM-1 and SCC-9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was subsequently analyzed by XTT and soft-agar colony assay. RESULTS: MMP-3 transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs (two of seven, 29%; P = 0.012). Both MMP-3 transcript and protein levels were significantly higher in OSCC masses than in neighboring tissues (P < 0.0001 and P = 0.04, respectively). Growth of the three cell lines was not affected, while the colony numbers of OECM-1 and CE81T/VGH were significantly reduced by the transfection of MMP-3 AODNs (P = 0.002 and P = 0.004, respectively). SCC-9 did not form colonies in soft-agar/medium. CONCLUSIONS: MMP-3 function may be required in most OSCCs, and it may support the anchorage-independent growth of both OSCC and esophageal carcinoma. ------>tmu_sno=None ------>sno=15187 ------>authors2=Liu YC ------>authors3=Huang WT ------>authors4=Huang GC ------>authors5=Hsiao JS ------>authors6=Chiang WF,Lee CH,Lin MH ------>authors6_c= ------>authors=Liu SY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Requirement of MMP-3 in anchorage-independent growth of oral squamous cell carcinomas. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=7 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=8 |