| Yeh SW |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.217 ------>paper_class3=2 ------>paper_class2=1 ------>vol=120 ------>confirm_bywho=cmbwrlee ------>insert_bywho=yehsw ------>Jurnal_Rank=31.3 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=75 ------>medlineContent= ------>unit=E0118 ------>insert_date=20070429 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=1521-6616 ------>authors_c= ------>score=89 ------>journal_name=Clin Immunol ------>paper_name=Pathogenic Human Monoclonal Antibody Against Desmoglein 3 ------>confirm_date=20070503 ------>tch_id=095046 ------>pmid=17532189 ------>page1=68 ------>fullAbstract=BACKGROUND: Although pemphigus vulgaris (PV)-IgG has been shown to activate urokinase plasminogen activator (uPA) in cultured keratinocytes, activation of uPA is thought to have no primary role in PV-acantholysis, because PV-IgG is still pathogenic in uPA- and tissue-PA-knockout mice. OBJECTIVE: To determine if PV-IgG-induced uPA activation is due to specific antibody against Dsg3, we examined whether or not pathogenic monoclonal anti-Dsg3 antibody can activate uPA, because PV-IgG is thought to contain antibodies against unknown antigens besides Dsg3. METHODS: We stimulated cultured normal human and DJM-1 keratinocytes with monoclonal anti-Dsg3 IgG1 antibodies (pathogenic AK23, AK19 and nonpathogenic AK18, AK20), negative control monoclonal mouse IgG1 and positive control PV-IgG. Cells were treated with IgGs over a time course of 24h, and uPA-protein content and activity in the culture medium were determined by enzyme-linked immunosorbent assay (ELISA) and chromogenic assay, respectively. RESULTS: The uPA-protein content in samples treated with or without pathogenic, nonpathogenic, control monoclonal mouse IgG1s and PV-IgGs increased continuously up to 24h, with no differences between samples, suggesting a spontaneous secretion. In contrast, uPA activity in the culture medium of cells treated with PV-IgG increased dramatically, whereas that of cells treated with all AK-IgGs and control monoclonal mouse IgG1 did not increase at all. CONCLUSION: These results suggest that PV-IgG-dependent uPA activation is not related to anti-Dsg3 antibody activity, which is an essential factor in PV-IgG acantholysis, and that it may be due to other antigens than Dsg3 or unknown factors contained in PV-IgG fraction. ------>tmu_sno=None ------>sno=15245 ------>authors2=Cavacini LA ------>authors3=Bhol KC ------>authors4=Lin MS ------>authors5=Kumar M ------>authors6=Duval M, [Posner MR, Ahmed AR] ------>authors6_c= ------>authors=Yeh SW ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=No activation of urokinase plasminogen activator by anti-desmoglein 3 monoclonal IgG antibodies in cultured human keratinocytes. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=1 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=7 |