Huang ZS |
------>authors3_c=??? ------>paper_class1=1 ------>Impact_Factor=5.854 ------>paper_class3=2 ------>paper_class2=1 ------>vol=278 ------>confirm_bywho=hsuan ------>insert_bywho=angela1975 ------>Jurnal_Rank=14.6 ------>authors4_c=??? ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=5693 ------>medlineContent= ------>unit=E0105 ------>insert_date=20070507 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c=??? ------>score=500 ------>journal_name=J. Biol. Chem. ------>paper_name=Selective strand annealing and selective strand exchange promoted by the N-terminal domain of hepatitis delta antigen. ------>confirm_date=20070826 ------>tch_id=095033 ------>pmid=12466279 ------>page1=5685 ------>fullAbstract=We have previously shown that the N-terminal domain of hepatitis delta virus (NdAg) has an RNA chaperone activity in vitro (Huang, Z. S., and Wu, H. N. (1998) J. Biol. Chem. 273, 26455-26461). Here we investigate further the basis of the stimulatory effect of NdAg on RNA structural rearrangement: mainly the formation and breakage of base pairs. Duplex dissociation, strand annealing, and exchange of complementary RNA oligonucleotides; the hybridization of yeast U4 and U6 small nuclear RNAs and of hammerhead ribozymes and cognate substrates; and the cis-cleavage reaction of hepatitis delta ribozymes were used to determine directly the role of NdAg in RNA-mediated processes. The results showed that NdAg could accelerate the annealing of complementary sequences in a selective fashion and promote strand exchange for the formation of a more extended duplex. These activities would prohibit NdAg from modifying the structure of a stable RNA, but allow NdAg to facilitate a trans-acting hammerhead ribozyme to find a more extensively matched target in cognate substrate. These and other results suggest that hepatitis delta antigen may have a biological role as an RNA chaperone, modulating the folding of viral RNA for replication and transcription. ------>tmu_sno=None ------>sno=15744 ------>authors2=Su WH ------>authors3=Wang JL ------>authors4=Wu HN ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Huang ZS ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=0 ------>updateTitle=Selective strand annealing and selective strand exchange promoted by the N-terminal domain of hepatitis delta antigen. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=8 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2003 ------>submit_flag=None ------>publish_month=2 |