| Au,L.C |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.687 ------>paper_class3=2 ------>paper_class2=1 ------>vol= ------>confirm_bywho=leehorng ------>insert_bywho=jjliu_96 ------>Jurnal_Rank=25.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=000 ------>insert_date=20070929 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=J.Biotechnol ------>paper_name=Secretory production of bioactive recombinant human granulocyte-macrophage colony-stimulating factor by a baculovirus expression system ------>confirm_date=20080418 ------>tch_id=096027 ------>pmid=8987879 ------>page1= ------>fullAbstract=The proliferation and differentiation of hematopoietic cells are stimulated by a group of glycoproteins called colony stimulating factors (CSFs). Previously, we found that the human hepatoma cell line HA22T/VGH secreted a high level of human granulocyte-macrophage colony-stimulating factor (hGM-CSF). The cDNA of hGM-CSF, including the signal peptide sequence, was amplified from the total RNA of HA22T/VGH by a reverse-transcription polymerase chain reaction and was cloned into the pUC18 vector. After confirming the nucleotide sequence, the cDNA was inserted into a pVL1393 baculovirus transfer vector. The recombinant baculovirus carrying hGM-CSF cDNA was generated by co-transfecting the hGM-CSF recombinant transfer vector and BaculoGold baculovirus DNA into the Sf9 insect cells. The expected hGM-CSF transcript was detected in the recombinant virus-infected Sf9 cells. The conditioned media of the infected cells were analyzed by a slot-blot immunoassay. The results indicate that the infected insect cells produced and secreted hGM-CSF. According to colony forming assay, a maximum titer of 2.1 x 10(6). U ml-1 of hGM-CSF in the medium was obtained on the third day after infection. ------>tmu_sno=None ------>sno=16012 ------>authors2=Liu,T.J., ------>authors3=Shen,H.D ------>authors4=Choo,K.B. ------>authors5=Wang,S.Y ------>authors6= ------>authors6_c= ------>authors=Au,L.C ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Secretory production of bioactive recombinant human granulocyte-macrophage colony-stimulating factor by a baculovirus expression system. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=1996 ------>submit_flag=None ------>publish_month=1 |