Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Jyh-Ming Chow
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------>ISSN=0301-472X
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------>journal_name=Experimental Hematology
------>paper_name=Downregulation of c-Myc determines the sensitivity to 2-methoxyestradiol-induced apoptosis in acute myeloid leukemia
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------>fullAbstract=OBJECTIVE: 2-Methoxyestradiol (2ME2) has been shown to induce apoptosis in leukemic cells, but its exact mechanism remains unclear. Because c-Myc plays a critical role in leukemogenesis, we evaluated whether 2ME2 acts on acute myeloid leukemia (AML) through modulation of c-Myc activity. MATERIALS AND METHODS: AML cell lines and primary AML leukemia were treated with 2ME2 and the relationship between 2ME2-induced apoptosis and changes in c-Myc activity was examined. RESULTS: 2ME2 induced mitochondrial apoptosis of human AML cells through increased reactive oxygen species. Further investigation showed that 2ME2 downregulated c-Myc expression in a time-dependent manner. Increased oxidative stress led to downregulation of c-Myc mRNA and protein, but did not affect the stability of c-Myc protein. To demonstrate the role of c-Myc in 2ME2-induced apoptosis, we ectopically expressed wild-type c-Myc in AML cells and found that ectopic expression of c-Myc abrogated the 2ME2-induced apoptosis. In addition, we showed that 2ME2 treatment inhibited phosphorylation of Akt and binding of nuclear factor-kappaB p65/p50 heterodimers to its DNA targets. As with results from cell lines studied, 2ME2 also induced cytotoxicity to primary AML cells and downregulated their c-Myc expression and induced apoptosis. CONCLUSION: Downregulation of c-Myc is critical for 2ME2-induced oxidative stress and apoptosis in AML cells. Our results might be extended to other types of cancers overexpressing c-Myc.
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------>authors2=Chien-Ru Liu
------>authors3=Che-Pin Lin
------>authors4=Chun-Nin Lee
------>authors5=Yun-Chih Cheng
------>authors6=Shufan Lin, H. Eugene Liu
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------>authors=Jyh-Ming Chow
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------>updateTitle=Downregulation of c-Myc determines sensitivity to 2-methoxyestradiol-induced apoptosis in human acute myeloid leukemia.
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------>no=2
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------>publish_year=2008
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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z