Huang TY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.956 ------>paper_class3=2 ------>paper_class2=1 ------>vol=148 ------>confirm_bywho=kueiru ------>insert_bywho=ptsai ------>Jurnal_Rank=21.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=180 ------>medlineContent= ------>unit=H0200 ------>insert_date=20080108 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Journal of Surgical Research ------>paper_name=HO-1 overexpression attenuates endotoxin effects on CAT-2 isozymes expression ------>confirm_date=20081124 ------>tch_id=091090 ------>pmid=18028947 ------>page1=172 ------>fullAbstract=BACKGROUND: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial mechanism that regulates nitric oxide (NO) production via inducible nitric oxide synthase (iNOS). We sought to investigate the effects of heme oxygenase-1 (HO-1) overexpression on CAT-2 isozymes, e.g., CAT-2, CAT-2A, and CAT-2B. MATERIALS AND METHODS: Adult male Sprague Dawley rats were allocated to receive lipopolysaccharide (LPS), normal saline, hemin (a HO-1 inducer), tin protoporphyrin (SnPP, a HO-1 inhibitor), LPS plus hemin, or LPS plus hemin plus SnPP. After maintaining for 6 h, rats were sacrificed and the expression and activity of individual enzyme was evaluated. RESULTS: LPS increased HO activity, HO-1 concentration, NO production, l-arginine transport, and concentrations of iNOS, CAT-2, and CAT-2B in rat lungs and kidney. LPS also increased HO activity, HO-1 concentration, NO production, l-arginine transport, and iNOS concentration but decreased CAT-2 and CAT-2B concentrations in rat liver. LPS increased CAT-2A concentration in rat liver but did not affect CAT-2A concentration in rat lungs and kidney. Hemin further increased HO activity and induced HO-1 overexpression in the lungs, kidney, and liver from LPS-treated rats. In addition, the effects of LPS on NO production, l-arginine transport, and concentrations of iNOS and CAT-2 isozymes were significantly attenuated by hemin. SnPP, on the other hand, reversed the effects of hemin. CONCLUSIONS: HO-1 overexpression significantly attenuates endotoxin-induced increases in NO production and l-arginine transport. Induction of HO-1 overexpression also significantly attenuates the effects of endotoxin on the expression of iNOS and CAT-2 isozymes in septic rats. ------>tmu_sno=None ------>sno=16609 ------>authors2=Tsai PS ------>authors3=Huang CJ ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Huang TY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=HO-1 overexpression attenuates endotoxin effects on CAT-2 isozymes expression. ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=2 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=8 |