| Wu HS |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=5.643 ------>paper_class3=2 ------>paper_class2=1 ------>vol=10 ------>confirm_bywho=leehorng ------>insert_bywho=wuhs ------>Jurnal_Rank=12.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=310 ------>medlineContent= ------>unit=E0310 ------>insert_date=20080205 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Emerg Infect Dis ------>paper_name=Serologic and molecular biologic methods for SARS-associated coronavirus infection, Taiwan. ------>confirm_date=20081008 ------>tch_id=093109 ------>pmid=15030702 ------>page1=304 ------>fullAbstract=Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase-polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR. With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus-based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection. ------>tmu_sno=None ------>sno=16640 ------>authors2=Chiu SC ------>authors3=Tseng TC ------>authors4=Lin SF ------>authors5=Lin JH ------>authors6=Hsu YF, Wang MC, Lin TL, Yang WZ, Ferng TL, Huang ------>authors6_c= ------>authors=Wu HS ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Serologic and molecular biologic methods for SARS-associated coronavirus infection, Taiwan. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2004 ------>submit_flag=None ------>publish_month=1 |