| Hu IC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.633 ------>paper_class3=2 ------>paper_class2=1 ------>vol=45 ------>confirm_bywho=amel ------>insert_bywho=chiueh ------>Jurnal_Rank=30.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=13 ------>paper_class2Letter=None ------>page2=7099 ------>medlineContent= ------>unit=G0100 ------>insert_date=20080313 ------>iam=4 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Biochemistry ------>paper_name=Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalytic bilin attachment. ------>confirm_date=20080313 ------>tch_id=092016 ------>pmid=16752899 ------>page1=7092 ------>fullAbstract=Allophycocyanin (APC) is one of the phycobiliproteins expressed in cyanobacteria. Phycobiliproteins contain a covalently bound chromophore, and thus, they are valuable as fluorescent probes. Biosynthesis of a functional phycobiliprotein is achieved by a bilin attachment process between the chromophore and apoprotein. Chromophore lyases are necessary to catalyze the chromophorylation of cyanobacterial phycobiliproteins, such as C-phycocyanin, and phycoerythrocyanin. To identify the lyase that catalyzes the chromophorylation of the APC alpha-subunit (ApcA), we searched the entire genomes of two cyanobacteria, Synechocystis sp. PCC6803 and Anabaena sp. PCC 7120; however, these genomes do not appear to encode an APC-specific chromophore lyase. In this study, chromophorylated ApcA (chromo-ApcA) was obtained via a spontaneous bilin attachment reaction. The absorption and fluorescence characteristics of chromo-ApcA were similar to those of the native APC alpha-subunit. The extent of chromophore attachment to apo-ApcA was comparable to that of the lyase-catalyzed reactions for other phycobiliproteins. These results indicate that ApcA has autocatalytic bilin:biliprotein lyase activity. ------>tmu_sno=None ------>sno=16778 ------>authors2=Lee TR ------>authors3=Lin HF ------>authors4=Chiueh CC ------>authors5=Lyu PC ------>authors6= ------>authors6_c= ------>authors=Hu IC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Biosynthesis of fluorescent allophycocyanin alpha-subunits by autocatalytic bilin attachment. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=23 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=6 |