| Cheng CJ |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.668 ------>paper_class3=2 ------>paper_class2=1 ------>vol=14 ------>confirm_bywho=jschu ------>insert_bywho=cjcheng ------>Jurnal_Rank=56.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=807 ------>medlineContent= ------>unit=E0102 ------>insert_date=20080321 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=1021-7770 ------>authors_c= ------>score=500 ------>journal_name=J Biomed Sci ------>paper_name=Aberrant expression and distribution of the OCT-4 transcription factor in seminomas ------>confirm_date=20080321 ------>tch_id=087010 ------>pmid=17682839 ------>page1=797 ------>fullAbstract=Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohistochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immunohistochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors. ------>tmu_sno=None ------>sno=17040 ------>authors2=Wu YC ------>authors3=Shu JA ------>authors4=Ling TY ------>authors5=Kuo HC ------>authors6=Wu JY, Chang EE, Chang SC, Huang YH ------>authors6_c= ------>authors=Cheng CJ ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Aberrant expression and distribution of the OCT-4 transcription factor in seminomas. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=6 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=11 |