| Lin CY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.468 ------>paper_class3=2 ------>paper_class2=1 ------>vol=42 ------>confirm_bywho=leehorng ------>insert_bywho=cylin ------>Jurnal_Rank=28.0 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=2 ------>paper_class2Letter=None ------>page2=367 ------>medlineContent= ------>unit=E0310 ------>insert_date=20080412 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Journal of Clinical Virology ------>paper_name=Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR ------>confirm_date=20081201 ------>tch_id=096028 ------>pmid=18455959 ------>page1=361 ------>fullAbstract=BACKGROUND: Type-specific persistence of human papillomavirus (HPV) infection can cause invasive cervical cancer. OBJECTIVES: To evaluate the efficacy of HPV detection and typing with a general polymerase chain reaction (PCR)-based genotyping array and to compare it with a type-specific PCR assay. STUDY DESIGN: Four hundred and thirty-three cervical samples were tested with a modified MY11/GP6+ PCR-based reverse-blot assay (EasyChip HPV Blot; King Car, Taiwan [hereafter HPV Blot]) and with 20 genotypes of L1-type-specific PCR (HPV-6, -11, -16, -18, -31, -33, -35, -39, -45, -51, -52, -53, -56, -58, -59, -62, -66, -68, -70, and -71 [CP8061]). RESULTS: The concordance of the two tests in determining HPV positivity was 96.8% (419/433), with a Cohen~s kappa=0.93 (95% CI: 0.90-0.97) and McNemar~s test of P=1.0, which indicates excellent agreement. The overall concordance of the two tests in the identification of type-specific HPV was 91.0% (394/433). Sensitivity (90-100%), specificity (99.2-100%), and accuracy (98.6-100%) rates of HPV Blot against the gold standard were satisfactory for HPV-16, -18, -58, -33, -52, -39, -45, -31, -51, -70 while HPV-71 (63.6%) had suboptimal sensitivity. Though the kappa values between the two tests for many individual genotypes could not be reliably calculated because of low positivity, the kappa values for HPV-16, -52, and -58 were excellent (0.93, 0.96, and 0.95, respectively). CONCLUSION: The modified MY11/GP6+ PCR-based HPV Blot assay is accurate and sensitive for detection and genotyping of HPV in cervical swab samples. ------>tmu_sno=None ------>sno=17486 ------>authors2=Chao Angel ------>authors3=Chou, HH ------>authors4=Yang YC ------>authors5=Ho CM ------>authors6=Lin RW , Chang TC, Chiou JY, Chao FY, Wang KL, Chi ------>authors6_c= ------>authors=Lin CY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=5 |