| Peng PH |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.263 ------>paper_class3=2 ------>paper_class2=1 ------>vol=115 ------>confirm_bywho=hsuan ------>insert_bywho=juansh ------>Jurnal_Rank=22.4 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=42 ------>medlineContent= ------>unit=E0105 ------>insert_date=20080417 ------>iam=4 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=448 ------>journal_name=Clin Sci (Lond). 2008 Mar 14; [Epub ahead of print] ------>paper_name=Heme oxygenase-1 gene transfer protects retinal ganglion cells from ischemia-reperfusion injury. ------>confirm_date=20081204 ------>tch_id=090103 ------>pmid=18341478 ------>page1=335 ------>fullAbstract=RGC (retinal ganglion cell) death following ischaemic insult is the major cause of a number of vision-threatening diseases, including glaucoma. The aim of the present study was to evaluate the role of HO-1 (haem oxygenase-1) in the retina against IR (ischaemia/reperfusion) injury. Adenovirus-mediated HO-1 gene transfer (Adv-HO-1) was carried out by injection into the vitreous body to induce HO-1 overexpression. At 3 weeks after transfection, levels of HO-1 expression, as measured by Western blot analysis, immunohistochemical staining and activity assay, were drastically up-regulated. Transient retinal ischaemia was induced by raising the intraocular pressure to 150 mmHg for 60 min. Untreated IR caused a significant decrease in RGC numbers at 3 and 7 days after reperfusion (76.1 and 67.2% of control eyes with sham IR respectively; P<0.001). Eyes pretreated with Adv-HO-1 had less RGC loss on day 3 and 7 following reperfusion compared with control eyes injected with Adv-GFP (adenovirus containing a gene for green fluorescent protein; 94.3 and 88.2% respectively; P=0.007 and 0.001). SnP (tin protoporphyrin), an HO-1 inhibitor, counteracted the effects of Adv-HO-1. In conclusion, these findings provide evidence that augmentation of HO-1 enzyme overexpression by intravitreal injection is able to protect RGCs against IR-induced damage. ------>tmu_sno=None ------>sno=17624 ------>authors2=Ko ML ------>authors3=Chen CF ------>authors4=Juan SH ------>authors5=Dec;115(11):335-42. ------>authors6= ------>authors6_c= ------>authors=Peng PH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Haem oxygenase-1 gene transfer protects retinal ganglion cells from ischaemia/reperfusion injury. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=11 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=12 |