| Wen-Ta Chiu |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=1 ------>paper_class2=1 ------>vol=40 ------>confirm_bywho=hj2166 ------>insert_bywho=wtchiu ------>Jurnal_Rank=None ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=288 ------>medlineContent= ------>unit=E0110 ------>insert_date=20080421 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=125 ------>journal_name=Chinese Medical Journal (Taipei). ------>paper_name=Neurogenic pulmonary edema. ------>confirm_date=20080423 ------>tch_id=073010 ------>pmid=19875734 ------>page1=283 ------>fullAbstract=BACKGROUND AND PURPOSE: We examined the effects of a caspase-1 inhibitor, N-Ac-Tyr-Val-Ala-Asp-chloromethyl ketone (Ac-YVAD-CMK), on neurogenic pulmonary edema in the endovascular perforation model of subarachnoid hemorrhage (SAH) in mice. METHODS: Ninety-seven mice were assigned to sham, SAH+vehicle, SAH+Ac-YVAD-CMK (6 or 10 mg/kg), and SAH+Z-Val-Ala-Asp-fluoromethylketone (Z-VAD-FMK, 6 mg/kg) groups. Drugs were intraperitoneally injected 1 hour post-SAH. Pulmonary edema measurements, Western blot for interleukin-1beta, interleukin-18, myeloperoxidase, matrix metalloproteinase (MMP)-2, MMP-9, cleaved caspase-3 and zona occludens-1, MMP zymography, terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling staining, and immunostaining were performed on the lung at 24 hours post-SAH. RESULTS: Ten- but not 6-mg/kg of Ac-YVAD-CMK significantly inhibited a post-SAH increase in the activation of interleukin-1beta and caspase-3 and the number of terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling-positive pulmonary endothelial cells, preventing neurogenic pulmonary edema. Another antiapoptotic drug, Z-VAD-FMK, also reduced neurogenic pulmonary edema. SAH did not change interleukin-18, myeloperoxidase, MMP-2, MMP-9, zona occludens-1 levels, or MMP activity. CONCLUSIONS: We report for the first time that Ac-YVAD-CMK prevents lung cell apoptosis and neurogenic pulmonary edema after SAH in mice. ------>tmu_sno=None ------>sno=17862 ------>authors2=Chun-Jen Shih ------>authors3=Hsin-Ying Chen ------>authors4=Lieh-Sheng Lin: ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Wen-Ta Chiu ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Caspase-1 Inhibitor Prevents Neurogenic Pulmonary Edema After Subarachnoid Hemorrhage in Mice. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=NULL ------>no=3 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=1987 ------>submit_flag=None ------>publish_month=1 |