| Tu CF |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=5.808 ------>paper_class3=2 ------>paper_class2=1 ------>vol=283 ------>confirm_bywho=jschu ------>insert_bywho=cjcheng ------>Jurnal_Rank=14.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=26 ------>paper_class2Letter=None ------>page2=12488 ------>medlineContent= ------>unit=E0102 ------>insert_date=20080505 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=0021-9258 ------>authors_c= ------>score=500 ------>journal_name=J Biol Chem. ------>paper_name=Domain and Functional Analysis of a Novel Platelet-Endothelial Cell Surface Protein, SCUBE1. ------>confirm_date=20081006 ------>tch_id=087010 ------>pmid=18303018 ------>page1=12478 ------>fullAbstract=SCUBE1 (signal peptide-CUB-EGF domain-containing protein 1) is a novel, secreted, cell surface glycoprotein expressed during early embryogenesis and found in platelet and endothelial cells. This protein is composed of an N-terminal signal peptide sequence followed by nine tandemly arranged epidermal growth factor (EGF)-like repeats, a spacer region, three cysteine-rich repeat motifs, and one CUB domain at the C terminus. However, little is known about its domain and biological function. Here, we generated a comprehensive panel of domain deletion constructs and a new genetic mouse model with targeted disruption of Scube1 (Scube1(Delta cub/Delta cub)) to investigate the domain function and biological significance. A number of cell-based assays were utilized to define the critical role of the spacer region for membrane association and establish that the EGF-like repeats 7-9 are sufficient for the formation of SCUBE1-mediated homophilic adhesions in a calcium-dependent fashion. Biochemical and molecular analyses showed that the C-terminal cysteine-rich motifs and CUB domain could directly bind and antagonize the bone morphogenetic protein activity. Furthermore, genetic ablation of this C-terminal region resulted in brain malformation in the Scube1(Delta cub/Delta cub) embryos. Together, our results support the dual roles of SCUBE1 on brain morphogenesis and cell-cell adhesions through its distinct domain function. ------>tmu_sno=None ------>sno=18409 ------>authors2=Yan YT ------>authors3=Wu SY ------>authors4=Djoko B ------>authors5=Tsai MT ------>authors6=Cheng CJ, Yang RB ------>authors6_c= ------>authors=Tu CF ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Domain and functional analysis of a novel platelet-endothelial cell surface protein, SCUBE1. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=18 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=2 |