| Chu PW, Yap MN, Wu CY, Huang CM, Pan FM, Tseng MJ, Chen ST |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=21 ------>confirm_bywho=cmbdshih ------>insert_bywho=cmbtseng ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=1745 ------>medlineContent= ------>unit=E0600 ------>insert_date=20000607 ------>iam=7 ------>update_date= ------>author=??? ------>change_event=5 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Electrophoresis ------>paper_name=A proteomic analysis of secreted proteins from xylan-induced Bacillus sp. strain K-1 ------>confirm_date=20010330 ------>tch_id=087001 ------>pmid=10870960 ------>page1=1740 ------>fullAbstract=The expression level of extracellular proteins in an alkaliphilic bacterium, Bacillus sp. strain K-1, grown in a xylan-containing medium, is significantly increased when compared with that grown in the nonxylan culture medium. A proteomic approach has been efficiently applied to separate and characterize these differentially expressed secretory proteins. Eight prominent protein spots were identified and subjected to N-terminal amino acid sequencing. The results show that three spots share considerable similarity with the xylanolytic enzymes and that two spots share considerable similarity with the GltC regulatory protein and 3-dehydroquinate dehydratase, respectively. In addition, the three other proteins show little similarity with the known proteins in the database. In conclusion, our results demonstrate that the proteomic approach is a highly efficient method to rapidly study the differential expression of the secreted proteins by Bacillus sp. strain K-1 grown under xylan-induced condition. ------>tmu_sno=None ------>sno=1853 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Chu PW, Yap MN, Wu CY, Huang CM, Pan FM, Tseng MJ, Chen ST ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=A proteomic analysis of secreted proteins from xylan-induced Bacillus sp. strain K-1. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho= ------>publish_year=2000 ------>submit_flag= ------>publish_month=None |