Yuan-Soon Ho |
------>authors3_c=??? ------>paper_class1=1 ------>Impact_Factor=2.146 ------>paper_class3=2 ------>paper_class2=1 ------>vol=44 ------>confirm_bywho=chenchho ------>insert_bywho=hoyuansn ------>Jurnal_Rank=19.6 ------>authors4_c=??? ------>comm_author= ------>patent_EDate=None ------>authors5_c=???? ------>publish_day=1 ------>paper_class2Letter=None ------>page2=556 ------>medlineContent= ------>unit=E0310 ------>insert_date=20080911 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c=??? ------>score=500 ------>journal_name=Journal Periodont Research ------>paper_name=Porphyromonas gingivalis fimbriae-dependent interleukin-6 autocrine regulation by increase of Gp130 in endothelial cells ------>confirm_date=20090805 ------>tch_id=084007 ------>pmid=19438975 ------>page1=550 ------>fullAbstract=BACKGROUND AND OBJECTIVE: Local persistent infection by Porphyromonas gingivalis leads to inflammatory systemic diseases, such as atherosclerosis. We have reported previously that avirulent P. gingivalis fimbriae-dependent invasion into endothelial cells might be involved in progression of atherosclerosis. Although interleukin-6 (IL-6) regulates progression of atherosclerosis, little is known about the relationship of P. gingivalis fimbriae-dependent invasion to IL-6 regulation in endothelial cells. MATERIAL AND METHODS: We examined the secretion of IL-6 and the expression of the IL-6 signal transducer gp130 in human umbilical vein endothelial cells (HUVEC) infected with the wild-type FDC381 strain of P. gingivalisand a fimbriae-deficient mutant (fimA) by enzyme-linked immunosorbent assay, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry (fluorescence-activated cell sorting, FACS) analysis. RESULTS: Coculture of HUVEC with P. gingivalis resulted in increase of IL-6 secretion at 24 h postinfection. Interestingly, the increase was inhibited significantly in HUVEC infected with the P. gingivalis fimA mutant. In addition, the increase of IL-6 secretion induced by P. gingivalis infection was significantly impaired by the meiosis specific kinase 1 inhibitor, PD98059, or the nuclear factor kappaB inhibitor, Bay11-7082. Furthermore, we demonstrated that gp130 expression increased with P. gingivalis infection. Importantly, gp130 expression was significantly impaired by P gingivalis fimA mutant infection compared with wild-type P. gingivalis infection, as assessed by both quantitative RT-PCR and FACS analysis. CONCLUSION: Our findings indicate that P. gingivalis fimbriae are important factors in the autocrine regulation of IL-6, by increasing gp130 in endothelial cells. ------>tmu_sno=None ------>sno=18810 ------>authors2=Ming-Tang Lai ------>authors3=Sin-Ju Liu ------>authors4=Cher-Tang Lin ------>authors5=Naruishi K ------>authors6=Takashiba S, Hsin-Hua Chou ------>authors6_c=??? ------>authors=Yuan-Soon Ho ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=0 ------>updateTitle=Porphyromonas gingivalis fimbriae-dependent interleukin-6 autocrine regulation by increase of gp130 in endothelial cells. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=1 |