| Huang SH |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.817 ------>paper_class3=2 ------>paper_class2=1 ------>vol=15 ------>confirm_bywho=cmshih ------>insert_bywho=cmlin ------>Jurnal_Rank=29.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=721 ------>medlineContent= ------>unit=E0103 ------>insert_date=20081018 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=0944-7113 ------>authors_c= ------>score=500 ------>journal_name=Phytomedicine ------>paper_name=Protective effects of Angelica sinensis extract on amyloid beta-peptide-induced neurotoxicity. ------>confirm_date=20081018 ------>tch_id=081006 ------>pmid=18448320 ------>page1=710 ------>fullAbstract=The protective effects of alcohol extract from the root of Angelica sinensis (AS) on beta-amyloid peptide (Abeta)-induced toxicity and the mechanism of these effects were investigated. Abeta is a pathological hallmark of Alzheimer~s disease; it decreased viability of Neuro 2A cells in a concentration-dependent manner with IC(50) of 14.9 microM. AS extract resulted in dose-dependent anti-Abeta toxicity according to MTT assay. Reactive oxygen species (ROS) analysis revealed a significant production of hydrogen peroxide, decreased glutathione (GSH) levels and increased lipid peroxidation (TBARS value) in the Abeta-treated Neuro 2A cells. The Abeta-treated cells also showed a significant decline in the mitochondrial transmembrane potential (DeltaPsim) and increase in the mitochondrial volume, and portions of the cytoplasm were sequestered by a membrane-bound vacuole. The malfunctions of Neuro 2A cells caused by Abeta were attenuated using AS extract. The AS extract protected cell viability against Abeta-induced oxidative damage (ROS, TBARS, and GSH contents) and rescued the DeltaPsim levels in a dose-dependent manner: the dosages of 25, 50, 100, and 200 microg/ml recovered 77%, 87%, 102%, and 105% of DeltaPsim, respectively. AS extract also recovered the enlarged mitochondria mass with dosages from 25 to 200 microg/ml. The results of this study demonstrated that AS extract possessed the activity to prevent the neurotoxicity induced by Abeta-associated oxidative stress, implying that AS has a potential role in the prevention of Alzheimer~s diseases. ------>tmu_sno=None ------>sno=18920 ------>authors2=Lin CM ------>authors3=Chiang BH ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Huang SH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Protective effects of Angelica sinensis extract on amyloid beta-peptide-induced neurotoxicity. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=9 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=9 |