| Lin CW |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=5.406 ------>paper_class3=2 ------>paper_class2=1 ------>vol=29 ------>confirm_bywho=hsuan ------>insert_bywho=juansh ------>Jurnal_Rank=14.4 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=15 ------>medlineContent= ------>unit=E0105 ------>insert_date=20081117 ------>iam=4 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Carcinogenesis. 2008 Sep;29(9):1807-15. Epub 2008 Jul 14. ------>paper_name=Quercetin inhibition of tumor invasion via suppressing PKC delta/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma cells. ------>confirm_date=20081123 ------>tch_id=090103 ------>pmid=18628248 ------>page1=1807 ------>fullAbstract=Quercetin (QUE; 3,5,7,3~,4~-tetrahydroxyflavone) has been shown to possess several beneficial biological activities including antitumor, anti-inflammation and antioxidant properties; however, the effects of QUE in preventing invasion by breast carcinoma cells are still undefined. Increases in the protein, messenger RNA and enzyme activity levels of matrix metalloproteinase (MMP)-9 were observed in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells, and these were blocked by QUE, but not by quercitrin or rutin. A translocation of protein kinase C (PKC)delta from the cytosol to the membrane followed by activation of extracellular signal-regulated kinase (ERK) and c-Jun/activator protein-1 (AP-1) by TPA was demonstrated, and TPA-induced MMP-9 activation and migration were inhibited by the pan PKC inhibitor, GF109203X, the specific PKCdelta inhibitor, rottlerin, an ERK inhibitor (PD98059) and an AP-1 inhibitor (curcumin). Application of QUE significantly suppressed TPA-induced activation of the PKCdelta/ERK/AP-1-signaling cascade. To elucidate the importance of hydroxyl (OH) substitutions to QUE~s inhibition of tumor migration, several structurally related flavones of QUE including 3~,4~-diOH, 3~,4~-diOCH(3), 3,5,7-triOH, 3,4~,4~-triOH, 3,3~,4~-triOCH(3), luteolin and fisetin were used. Results suggested that OH groups at both C3~ and C4~ play central roles in QUE~s inhibition of TPA-induced MMP-9 activation and migration, and an additional OH at C3, C5 or C7 may increase the inhibitory potency of the 3~,4~-diOH flavone against TPA-induced MMP-9 activity and migration. The antitumor invasion and migration effects of breast carcinoma cells induced by QUE with the structure-activity relationship analysis were identified. ------>tmu_sno=None ------>sno=18990 ------>authors2=Hou WC ------>authors3=Shen SC ------>authors4=Juan SH ------>authors5=Ko CH ------>authors6=Wang LM, Chen YC ------>authors6_c= ------>authors=Lin CW ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Quercetin inhibition of tumor invasion via suppressing PKC delta/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma cells. ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=9 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=9 |