| Chien-Che Wang |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.319 ------>paper_class3=2 ------>paper_class2=1 ------>vol=53 ------>confirm_bywho=shiemin ------>insert_bywho=wtchiu ------>Jurnal_Rank=82.0 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=563 ------>medlineContent= ------>unit=E0110 ------>insert_date=20081120 ------>iam=5 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=466 ------>journal_name=Dig Dis Sci ------>paper_name=?-Melanocyte-Stimulating Hormone Gene Transfer Attenuates Inflammation after Bile Duct Ligation in the Rat. ------>confirm_date=20081202 ------>tch_id=073010 ------>pmid=17676395 ------>page1=556 ------>fullAbstract=Cholestasis occurs in a wide variety of human liver diseases, and hepatocellular injury is an invariant feature of cholestasis causing liver dysfunction and inflammation, promoting fibrogenesis, and ultimately leading to liver failure. alpha-Melanocyte-stimulating hormone (alpha-MSH) is a potent anti-inflammatory agent in many models of inflammation, suggesting that it inhibits a critical step common to different forms of inflammation. The aim of this study was to investigate whether the gene transfer of alpha-MSH could attenuate hepatic inflammation after bile duct ligation in the rat. Studies were performed in bile duct-ligated (BDL) rats. Hydrodynamic-based gene transfection with alpha-MSH plasmid via rapid tail vein injection was performed 30 min after ligation of bile duct. The endpoints were studied as markers of inflammation 7 days after bile duct ligation. alpha-MSH expression in liver via a single administration of naked plasmid was demonstrated. Liver inflammation index, including neutrophil infiltration and serum alanine aminotransferase, were significantly reduced in alpha-MSH gene transfer rats. Markers for liver inflammation, including expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and inducible NO synthase (iNOS) mRNA, as assessed by real-time PCR, were also attenuated by alpha-MSH gene therapy. Expression of iNOS protein in liver diminished after alpha-MSH gene transfer. Consistent with these data, hepatic stellate cells (HSC) and Kupffer cells were markedly inhibited in alpha-MSH gene-treated rats. Our findings show that gene transfer of alpha-MSH could attenuate hepatic inflammation after bile duct ligation in the rat. ------>tmu_sno=None ------>sno=19524 ------>authors2=Jia-Wei Lin ------>authors3=Liang-Ming Lee ------>authors4=Chien-Min Lin ------>authors5=Wen-Ta Chiu ------>authors6=Hsin-Te Pai, Kuo-Sheng Hung ------>authors6_c= ------>authors=Chien-Che Wang ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=alpha-melanocyte-stimulating hormone gene transfer attenuates inflammation after bile duct ligation in the rat. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=2 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=1 |