Hsu SC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=8.115 ------>paper_class3=2 ------>paper_class2=1 ------>vol=119 ------>confirm_bywho=cmbwrlee ------>insert_bywho=tsai5326 ------>Jurnal_Rank=5.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1528 ------>medlineContent= ------>unit=E0118 ------>insert_date=20081125 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=487 ------>journal_name=J Allergy Clin Immunol ------>paper_name=Antigen Coupled with Lewis-x Trisaccharides Elicits Potent Immune Response in mice ------>confirm_date=20081127 ------>tch_id=090128 ------>pmid=17353042 ------>page1=1522 ------>fullAbstract=BACKGROUND: Glycoproteins containing Lewis-x (Le(x)) trisaccharides are often associated with the host~s adaptive T(H)2-type immunity, but the mechanisms underlying the T(H)2-biased response are at present unclear. OBJECTIVE: The modulatory effect of Le(x) or its glycoconjugates on IgE/T(H)2 responses was investigated. METHODS: The levels of serum antibodies and cytokines were analyzed by means of ELISA, RT-PCR, or both. RESULTS: In C3H mice Le(x) coupled with BSA (Le(x)-BSA) elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic T(H)2 cytokines when compared with those seen in BSA-sensitized mice. In BALB/c mice sensitized with Le(x)-BSA or Le(x) mixed with ovalbumin, significantly increased levels of specific IgE and IgG2a antibodies were found concomitant with reduced levels of serum IL-12p70. These effects were attenuated in IL-12-deficient BALB/c mice. Le(x) and an isomer, Le(y), but not other isomers, inhibited the production of LPS-induced IL-12p70, associated with a significant reduction of nuclear NF-kappaB, in bone marrow-derived dendritic cells from BALB/c mice, suggesting that Le(x)-induced suppression of IL-12p70 results in an enhanced T(H)2 response. The addition of mannan, a known ligand for dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin, abrogated the suppressive effect of Le(x) trisaccharides. CONCLUSION: These results provide evidence for a potential role of Le(x) trisaccharides in shaping the immune responses through, at least in part, its suppressive effect on IL-12p70 production. Considering the relative ubiquity of glycoproteins with Le(x) or similar oligosaccharides, including plant-derived (or food-derived) allergens, these findings might have a broad implication. CLINICAL IMPLICATIONS: The adjuvant activity of Le(x) trisaccharides might aid in vaccine design and might be important in determining the allergenicity of proteins containing this or other similar structures. ------>tmu_sno=None ------>sno=19824 ------>authors2=Tsai TH ------>authors3=Kawasaki H ------>authors4=Chen CH ------>authors5=Plunkett B ------>authors6=Lee RT, Huang SK ------>authors6_c= ------>authors=Hsu SC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=1 |