Huang YH |
------>authors3_c=??? ------>paper_class1=1 ------>Impact_Factor=5.045 ------>paper_class3=2 ------>paper_class2=1 ------>vol=147 ------>confirm_bywho=None ------>insert_bywho=rita1204 ------>Jurnal_Rank=17.4 ------>authors4_c=??? ------>comm_author= ------>patent_EDate=None ------>authors5_c=??? ------>publish_day=1 ------>paper_class2Letter=None ------>page2=4800 ------>medlineContent= ------>unit=E0103 ------>insert_date=20081128 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c=??? ------>score=436 ------>journal_name=Endocrinology. ------>paper_name=Localization and characterization of an orphan receptor, guanylyl cyclase-G, in mouse testis and spermatozoa. ------>confirm_date=None ------>tch_id=090143 ------>pmid=16857755 ------>page1=4792 ------>fullAbstract=We recently identified a novel testis-enriched receptor guanylyl cyclase (GC) in the mouse, designated mGC-G. To further investigate its protein expression and function, we generated a neutralizing antibody specifically against the extracellular domain of this receptor. RT-PCR and immunohistochemical analyses show that mGC-G is predominantly expressed from round spermatids to spermatozoa in mouse testis at both the mRNA and protein levels. Flow cytometry and confocal immunofluorescence reveal that mGC-G is a cell surface protein restricted to the plasma membrane overlying the acrosome and midpiece of the flagellum in mature sperm. Interestingly, Western blot analysis demonstrates that testicular mGC-G is approximately 180 kDa but is subject to limited proteolysis during epididymal sperm transport, resulting in a smaller fragment tethered on the mature sperm surface. On Fluo-3 cytometrical analysis and computer-assisted sperm assay, we found that serum albumin-induced elevation of sperm intracellular Ca(2+) concentration, protein tyrosine phosphorylation, and progressive motility associated with capacitation are markedly reduced by preincubation of the anti-mGC-G neutralizing antibody. Together, these results indicate that mGC-G is proteolytically modified in mature sperm membrane and suggest that mGC-G-mediated signaling may play a critical role in gamete/reproductive biology. ------>tmu_sno=None ------>sno=20040 ------>authors2=Wei CC ------>authors3=Su YH ------>authors4=Wu BT ------>authors5=Ciou YY ------>authors6=Tu CF, Cooper TG, Yeung CH, Chu ST, Yang RB ------>authors6_c=??????? ------>authors=Huang YH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=0 ------>updateTitle=Localization and characterization of an orphan receptor, guanylyl cyclase-G, in mouse testis and sperm. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=10 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2006 ------>submit_flag=None ------>publish_month=7 |