| Hung, WY |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.382 ------>paper_class3=2 ------>paper_class2=1 ------>vol=23 ------>confirm_bywho=lm ------>insert_bywho=lm ------>Jurnal_Rank=28.8 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=142 ------>medlineContent= ------>unit=E0117 ------>insert_date=20081206 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=MUTAGENESIS. ------>paper_name=Tandem duplication/triplication correlated with poly-cytosine stretch variation in human mitochondrial DNA D-loop region. ------>confirm_date=20081212 ------>tch_id=090105 ------>pmid=18252697 ------>page1=137 ------>fullAbstract=Somatic mutations in the mitochondrial DNA (mtDNA) displacement loop (D-loop) region have been frequently detected in various human cancers. In a previous study, we identified a polyplasmic 260-bp tandem duplication and triplication mutation in the mtDNA D-loop of one gastric cancer. In the present study, we adopted a more sensitive back-to-back polymerase chain reaction method to screen for this 260-bp tandem duplication/triplication in 197 cancers and their adjacent non-cancerous tissues. Nine samples of primary cancer (4.6%) were found to harbor the tandem duplication/triplication and these were made up of four out of 31 (12.9%) gastric cancers, two out of 45 (4.4%) breast cancers, two out of 56 (3.6%) hepatocellular cancers and one out of 32 (3.1%) colon cancers, but no tandem duplication/triplication was present in any of 33 lung cancers. We also found an expanded and polyplasmic poly-cytosine (poly-C) stretch around nucleotide position (np) 568 in eight of the 197 (4.1%) cancer patients. All the eight cancer samples carried the 260-bp tandem duplication/triplication. In addition, we detected the np 568 poly-C length variations in 11 of 234 (4.7%) peripheral blood samples of non-cancer population and the 260-bp tandem duplication in nine of the 11 cases with the np 568 poly-C length variations. These observations suggest that the occurrence of the tandem duplication/triplication in mtDNA D-loop is not specific for cancer tissues, but highly associated with the poly-C length variations around np 568. ------>tmu_sno=None ------>sno=20553 ------>authors2=Lin, JC ------>authors3=Lee, LM ------>authors4=Wu, CW ------>authors5=Tseng, LM ------>authors6=Yin, PH; Chi, CW; Lee, HC* ------>authors6_c= ------>authors=Hung, WY ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Tandem duplication/triplication correlated with poly-cytosine stretch variation in human mitochondrial DNA D-loop region. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=2 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=1 |