| Lin MH |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=4.657 ------>paper_class3=2 ------>paper_class2=1 ------>vol=4 ------>confirm_bywho=seanlee ------>insert_bywho=syliu ------>Jurnal_Rank=23.7 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=968 ------>medlineContent= ------>unit=F0100 ------>insert_date=20081208 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Autophage ------>paper_name=Autophagy induction by a natural ingredient of areca nut ------>confirm_date=20081208 ------>tch_id=078008 ------>pmid=18758233 ------>page1=967 ------>fullAbstract=We recently identified an autophagy-inducing areca nut ingredient (AIAI) in the partially purified 30-100 kDa fraction of areca nut extract (ANE), designated as ANE 30-100K. Before disintegration, most ANE 30-100K-treated cells exhibit rounding morphology, cytoplasmic clearance, and nuclear shrinkage, distinct from arecoline- and cisplatin-induced cellular apoptosis. This unique death pattern is verified to be autophagy by LC3-I cleavage, acidic vesicles, and autophagic vacuoles. As analyzed by Molish~s Test, Selinowaff~s Test, and thin-layer chromatography, most of the ANE 30-100K constituents are carbohydrates, whereas the protein content of this fraction is less than 1% as assessed by protein assay reagent. The cytotoxicity of ANE 30-100K is further shown to be sensitive to cellulase and proteinase K digestion suggesting AIAI in ANE 30-100K to be a proteoglycan (or glycoprotein). Thus, although ANE contains apoptosis-inducing ingredients such as arecoline, it predominantly triggers autophagic cell death by this natural AIAI. ------>tmu_sno=None ------>sno=20575 ------>authors2=Liu Sy ------>authors3=Liu YC ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Lin MH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Autophagy induction by a natural ingredient of areca nut. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=7 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=1 |