Hsu N |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.301 ------>paper_class3=2 ------>paper_class2=1 ------>vol=14 ------>confirm_bywho=None ------>insert_bywho=pclin ------>Jurnal_Rank=17.4 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1261 ------>medlineContent= ------>unit=000 ------>insert_date=20081208 ------>iam=5 ------>update_date=None ------>author=??? ------>change_event=1 ------>ISSN= ------>authors_c= ------>score=8 ------>journal_name=Journal of Clinical Nursing ------>paper_name=A compari ------>confirm_date=None ------>tch_id=097007 ------>pmid=18967119 ------>page1=1260 ------>fullAbstract=An algorithm is proposed for the estimation of binding parameters for the interaction of biologically important macromolecules with smaller ones from electrometric titration data. The mathematical model is based on the representation of equilibria in terms of probability concepts of statistical molecular thermodynamics. The refinement of equilibrium concentrations of the components and estimation of binding parameters (log site constant and cooperativity factor) is performed using singular value decomposition, a chemometric technique which overcomes the general obstacles due to near singularity. The present software is validated with a number of biochemical systems of varying number of sites and cooperativity factors. The effect of random errors of realistic magnitude in experimental data is studied using the simulated primary data for some typical systems. The safe area within which approximate binding parameters ensure convergence has been reported for the non-self starting optimization algorithms. ------>tmu_sno=None ------>sno=20590 ------>authors2=Chen BTH ------>authors3=Lee LL ------>authors4=Chung MH ------>authors5=Lin PC ------>authors6= ------>authors6_c= ------>authors=Hsu N ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=A software for the estimation of binding parameters of biochemical equilibria based on statistical probability model. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=10 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=1 |