| Tsou TL |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.549 ------>paper_class3=2 ------>paper_class2=1 ------>vol= ------>confirm_bywho=hj2166 ------>insert_bywho=hj2166 ------>Jurnal_Rank=55.7 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0110 ------>insert_date=20081209 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=493 ------>journal_name=JAOAC accepted ------>paper_name=Development of a new HPLC method for the simultaneous determination of ticarcillin and clavulanic acid in pharmaceutical formulation ------>confirm_date=20081229 ------>tch_id=094125 ------>pmid=19714977 ------>page1= ------>fullAbstract=A new HPLC method has been developed and validated for the simultaneous determination of ticarcillin (TIC) and clavulanic acid (CA) in pharmaceutical formulations. The HPLC separation was achieved on a beta-cyclodextrin column (Cyclobond I, 250 x 4.6 mm, 5 microm) with methanol-16 mM pH 6.0 ammonium acetate buffer (50 + 50, v/v) mobile phase at a flow rate of 0.8 mL/min. Detection was at 220 nm. Validation of the method was performed by evaluating specificity, robustness, accuracy, and precision. The calibration curves were linear in the range of 1-100 microg/mL for CA and 2-200 microg/mL for TIC. The LOQs based on the standard regression lines were 0.42 and 1.42 microg/mL for CA and TIC, respectively, and the LOD were 0.14 and 0.47 microg/mL, respectively. Total recoveries of synthetic mixtures (CA:TIC = 1:10, 1:15, and 1:30) were 99.25-100.99% for CA and 99.54-100.82% for TIC. Compared with the U.S. Pharmacopeia method, the proposed method has the advantage of a relatively low flow rate and short analysis time. The proposed method was successfully applied for the simultaneous determination of these two drugs in sterilized H20 and 5% dextrose injection solutions. ------>tmu_sno=None ------>sno=20657 ------>authors2=Lee CW ------>authors3=Wang HJ ------>authors4=Cheng YC ------>authors5=Liu YT ------>authors6=Chen SH ------>authors6_c= ------>authors=Tsou TL ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Development of a new HPLC method for the simultaneous determination of ticarcillin and clavulanic acid in pharmaceutical formulations. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=1 |